Integration of multiple components in polystyrene-based microfluidic devices part I: fabrication and characterization.
Integration of multiple components in polystyrene-based microfluidic devices part I: fabrication and characterization.
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DOI：
10.1039/c2an36168j
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发表时间：
2013-01-07
期刊：
影响因子：
--
通讯作者：
Martin RS
中科院分区：
文献类型：
--
作者：
Johnson AS;Anderson KB;Halpin ST;Kirkpatrick DC;Spence DM;Martin RS
在由两部分组成的系列的第一部分中，我们描述了一种简单且廉价的制造聚苯乙烯器件的方法，该方法基于在 PDMS 模具或电极材料周围熔化聚苯乙烯（来自培养皿或粉末形式）。首先描述了将微通道合并到聚苯乙烯中并将所得装置与标准实验室设备集成的能力，例如用于读出分析物的光学读板器和用于流体推进的微量移液器。详细介绍了使用标准多通道微量移液器和基于 PDMS 的注射块将样品和试剂输送到设备通道的简单方法。微流体装置与这些片外功能（样品传输和读出）的集成可实现高通量筛选和分析。还演示了一种制造带有嵌入式电极的聚苯乙烯基装置的方法，从而通过使用钯电极（用于解耦器）和碳纤维束（用于检测）将微芯片电泳与电化学检测集成。该装置密封在基于 PDMS 的微通道上，用于多巴胺、肾上腺素、儿茶酚和 3,4-二羟基苯乙酸的电泳分离和电流检测。最后，将这些装置与基于 PDMS 的微芯片在光学透明度和抗血小板药物氯吡格雷的吸收方面进行比较。本系列的第一部分为第二部分奠定了基础，其中这些设备用于各种片上细胞分析。
In Part I of a two-part series, we describe a simple, and inexpensive approach to fabricate polystyrene devices that is based upon melting polystyrene (from either a Petri dish or powder form) against PDMS molds or around electrode materials. The ability to incorporate microchannels in polystyrene and integrate the resulting device with standard laboratory equipment such as an optical plate reader for analyte readout and micropipettors for fluid propulsion is first described. A simple approach for sample and reagent delivery to the device channels using a standard, multi-channel micropipette and a PDMS-based injection block is detailed. Integration of the microfluidic device with these off-chip functions (sample delivery and readout) enables high throughput screens and analyses. An approach to fabricate polystyrene-based devices with embedded electrodes is also demonstrated, thereby enabling the integration of microchip electrophoresis with electrochemical detection through the use of a palladium electrode (for a decoupler) and carbon-fiber bundle (for detection). The device was sealed against a PDMS-based microchannel and used for the electrophoretic separation and amperometric detection of dopamine, epinephrine, catechol, and 3,4-dihydroxyphenylacetic acid. Finally, these devices were compared against PDMS-based microchips in terms of their optical transparency and absorption of an anti-platelet drug, clopidogrel. Part I of this series lays the foundation for Part II, where these devices were utilized for various on-chip cellular analysis.
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