Functional and pharmacological analysis of cardiomyocytes differentiated from human peripheral blood mononuclear-derived pluripotent stem cells.
Functional and pharmacological analysis of cardiomyocytes differentiated from human peripheral blood mononuclear-derived pluripotent stem cells.
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DOI：
10.1016/j.stemcr.2014.04.017
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发表时间：
2014-07-08
影响因子：
5.9
通讯作者：
Benjamin, Ivor J.
中科院分区：
文献类型：
--
作者：
Riedel, Michael;Jou, Chuanchau J.;Lai, Shuping;Lux, Robert L.;Moreno, Alonso P.;Spitzer, Kenneth W.;Christians, Elizabeth;Tristani-Firouzi, Martin;Benjamin, Ivor J.
诱导多能干细胞 (iPSC) 技术的进步为常规衍生患者和疾病特异性人类 iPSC 心肌细胞 (CM) 模型奠定了基础，用于临床前药物筛选和个性化医疗方法。外周血单核细胞 (PBMC) 是体细胞的有利来源，因为它们易于获得且易于转导。在这里，我们使用膜片钳、钙瞬态和多电极阵列 (MEA) 分析发现，PBMC 衍生的 iPSC CM 的电生理特性和药理学反应通常与其他体细胞衍生的 iPSC CM 相似。来自同一患者的不同 iPSC 系表现出相似的电生理特征和药理学反应。最后，我们证明人类 iPSC CM 在响应快速电刺激时会发生钙处理特性和基因表达的急剧变化，为研究人类快速心律失常的体外快速起搏模型系统奠定了基础。外周血单核细胞是体细胞的有利来源 外周血单核干细胞来源的心肌细胞的功能分析 基于 MEA 的激活恢复间隔稳健地跟踪复极 基于干细胞的快速起搏模型用于研究人类快速心律失常 干细胞技术的进步允许常规推导患者和疾病特异性心肌细胞 (CM) 模型。 Benjamin、Tristani-Firouzi 及其同事在此报告了从外周血单核细胞衍生的多能干细胞分化而来的 CM 的功能和药理学特性。 CM 响应快速电刺激，在钙处理特性和基因表达方面发生急剧变化，为人类快速心律失常的研究奠定了基础。
Advances in induced pluripotent stem cell (iPSC) technology have set the stage for routine derivation of patient- and disease-specific human iPSC-cardiomyocyte (CM) models for preclinical drug screening and personalized medicine approaches. Peripheral blood mononuclear cells (PBMCs) are an advantageous source of somatic cells because they are easily obtained and readily amenable to transduction. Here, we report that the electrophysiological properties and pharmacological responses of PBMC-derived iPSC CM are generally similar to those of iPSC CM derived from other somatic cells, using patch-clamp, calcium transient, and multielectrode array (MEA) analyses. Distinct iPSC lines derived from a single patient display similar electrophysiological features and pharmacological responses. Finally, we demonstrate that human iPSC CMs undergo acute changes in calcium-handling properties and gene expression in response to rapid electrical stimulation, laying the foundation for an in-vitro-tachypacing model system for the study of human tachyarrhythmias. Peripheral blood mononuclear cells are an advantageous source of somatic cells Functional analysis of peripheral blood mononuclear stem cell-derived cardiac cells MEA-based activation-recovery interval robustly tracks repolarization Stem cell-based tachypacing model for the study of human tachyarrhythmias Advances in stem cell technology allow for routine derivation of patient- and disease-specific cardiomyocyte (CM) models. Here, Benjamin, Tristani-Firouzi, and colleagues report the functional and pharmacological properties of CMs differentiated from peripheral blood mononuclear cell-derived pluripotent stem cells. CMs undergo acute changes in calcium-handling properties and gene expression in response to rapid electrical stimulation, laying the foundation for the study of human tachyarrhythmias.
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DOI：
10.1007/978-1-62703-511-8_7
发表时间：
2013
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Churko JM;Burridge PW;Wu JC
通讯作者：
Wu JC
影响因子：
4.8
作者：
KATO, R;VENKATESH, N;SINGH, BN
通讯作者：
SINGH, BN
影响因子：
64.8
作者：
Itzhaki, Ilanit;Maizels, Leonid;Gepstein, Lior
通讯作者：
Gepstein, Lior
影响因子：
37.8
作者：
Fuller, MS;Sándor, G;Lux, RL
通讯作者：
Lux, RL
影响因子：
5
作者：
Brundel, Bianca J. J. M.;Henning, Robert H.;Kampinga, Harm H.
通讯作者：
Kampinga, Harm H.