Integration of Enzymatic Catalysts in a Reactive Distillation Column with Structured Packings
Integration of Enzymatic Catalysts in a Reactive Distillation Column with Structured Packings
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将酶催化剂集成到带有规整填料的反应蒸馏塔中
DOI：
10.1021/ie300837v
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发表时间：
2012
影响因子：
4.2
通讯作者：
I. Smirnova
中科院分区：
文献类型：
--
作者：
R. Heils;A. Sont;P. Bubenheim;A. Liese;I. Smirnova
反应精馏中反应和分离的一体化流程设置有利于酶催化的反应，因为酶催化的反应往往受到反应产物的平衡限制和/或抑制。然而，到目前为止，还没有合适的方法来描述将酶落实到蒸馏塔中。在这项工作中，开发了一种特殊类型的生物催化涂层，用于商业上可用的规整填料，以实现反应蒸馏塔中的酶反应。脂肪酶Calb被固定在硅胶基涂层中，表现出很高的长期稳定性，即涂层可以在室温下保存至少50天，而不会造成任何酶活性的显著损失。此外，硅胶涂层在有机介质中和温度升高时都是稳定的。反应精馏实验在间歇反应精馏装置中进行，该装置包括来自Montz(德国希尔登)的结构化A3-500填料，包覆有催化硅胶。与标准间歇反应器中的相应装置相比，分步脱除馏分油中的低沸点产物使平衡向产物侧移动，使反应物的转化率从60%提高到98%。考察了硅胶涂层的稳定性和蒸馏过程中酶的损失。总体而言，将酶反应集成到反应蒸馏塔中为生物催化剂在有机合成中的应用提供了新的可能性。
The integrated process setup of reaction and separation in reactive distillation can be favorable for enzyme-catalyzed reactions that are often equilibrium-limited and/or inhibited by the product of the reaction. However, until now, no appropriate way has described the implementation of the enzymes into distillation columns. In this work, a special type of biocatalytic coating for commercially available structured packings was developed to enable enzymatic reactions in reactive distillation columns. Lipase CALB was immobilized within the silica-gel based coating and showed high long-term stability, i.e., the coatings can be stored for at least 50 days at room temperature without any significant loss of enzyme activity. In addition, the silica-gel coatings were stable in organic media and at increased temperatures. Reactive distillation experiments were carried out in a batch reactive distillation setup comprising of structured A3-500 packings from Montz (Hilden, Germany) coated with the catalytic silica gel. Stepwise removal of the low boiling point product from the distillate shifted the equilibrium to the product side and increased the conversion of the reactants from 60% to 98%, in comparison with the corresponding setup in a standard batch reactor. The stability of the silica-gel coating and loss of the enzyme was investigated during the distillation runs. Overall, the integration of an enzymatic reaction into a reactive distillation column offers new possibilities for the application of biocatalysts in organic synthesis.
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