Characterization of the carbohydrate moiety of Clerodendron trichotomum lectins. Its structure and reactivity toward plant lectins.
Characterization of the carbohydrate moiety of Clerodendron trichotomum lectins. Its structure and reactivity toward plant lectins.
复制标题
Clerodendron trichotomum 凝集素碳水化合物部分的表征。
DOI：
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Y. Arata
中科院分区：
文献类型：
--
作者：
H. Kitagaki;I. Matsumoto;N. Seno;N. Takahashi;S. Endo;Y. Arata
采用内酰胺- sepharose亲和层析的方法，从毛毛栎（Clerodendron trichotomum）果实和叶片中分离到凝集素。纯化的凝集素（C. trichotomum agglutinin: CTA）在SDS/聚丙烯酰胺凝胶电泳上均相，并用物理化学和免疫化学方法对其碳水化合物部分进行了表征。以水果CTA中提取的n-寡糖糖肽酶（杏仁，EC 3.5.1.52）处理后释放天冬酰胺寡糖，采用凝胶过滤和薄层色谱分离得到天冬酰胺寡糖。通过高效液相色谱、糖分析和1H-NMR谱分析，确定了优势低聚糖的结构为Xyl β 1----2 (Man α 1----6)(Man α 1----3)Man β 1----4GlcNAc β 1----4(Fuc α 1----3)GlcNAc。研究了CTA碳水化合物部分对各种凝集素的反应性。将果实和叶片cta应用于聚丙烯酰胺凝胶电泳，转移到硝化纤维素片上，并用辣根过氧化物酶偶联凝集素进行检测。豆豆蛋白A、扁豆凝集素、豌豆凝集素、蚕豆凝集素和欧洲镰刀凝集素I与果实CTA结合，但小麦胚芽凝集素不与CTA结合。结果表明这些植物凝集素具有新的结合特性：在n -连接低聚糖的-甘露糖基残基的C-2取代的-木糖基残基不影响与甘露糖特异性凝集素的结合，扁豆、豌豆和蚕豆凝集素可以与含有α -l -聚焦基残基的n -连接低聚糖的天冬酰胺-乙酰- d -氨基葡萄糖残基的C-3连接的n -连接低聚糖结合，而欧洲Ulex凝集素I可以与n -连接低聚糖的（α 1----3）连接的聚焦基结合。
Lectins were isolated from fruits and leaves of Clerodendron trichotomum by affinity chromatography on lactamyl-Sepharose. The purified lectins (C. trichotomum agglutinin: CTA) were homogeneous on SDS/polyacrylamide gel electrophoresis, and the carbohydrate moiety was characterized by physicochemical and immunochemical methods. The asparagine-linked oligosaccharides were released by treatment with N-oligosaccharide glycopeptidase (almond, EC 3.5.1.52) of peptic glycopeptides obtained from fruit CTA, and separated by gel filtration and thin-layer chromatography. The structure of the predominant oligosaccharide was determined as Xyl beta 1----2 (Man alpha 1----6)(Man alpha 1----3)Man beta 1----4GlcNAc beta 1----4(Fuc alpha 1----3)GlcNAc by high-performance liquid chromatography, sugar analysis and 1H-NMR spectroscopy. The reactivity of the carbohydrate moiety of CTA toward various lectins was studied. Fruit and leaf CTAs were applied to polyacrylamide gel electrophoresis, transferred to nitrocellulose sheets and detected with horseradish-peroxidase-conjugated lectins. Concanavalin A, lentil lectin, pea lectin, Vicia faba lectin and Ulex europeus agglutinin I, but not wheat germ lectin, bound to fruit CTA. The results indicate new binding properties of these plant lectins: a beta-xylosyl residue substituted at C-2 of the beta-mannosyl residue of N-linked oligosaccharide does not affect the binding with mannose-specific lectins, lentil, pea and Vicia faba lectins can bind to N-linked oligosaccharides containing an alpha-L-fucosyl residue attached to C-3 of the asparagine-linked N-acetyl-D-glucosamine residue, and Ulex europeus agglutinin I can bind to the (alpha 1----3)-linked fucose residue of the N-linked oligosaccharide.
双花豆茎叶中凝集素样糖蛋白的碳水化合物结合活性。
DOI：
10.1016/0006-291x(80)91185-7
发表时间：
1980
影响因子：
3.1
作者：
Etzler,ME;Borrebaeck,C
通讯作者：
Borrebaeck,C
DOI：
10.1016/s0021-9258(19)69037-x
发表时间：
1981-07
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
K. Kornfeld;M. Reitman;R. Kornfeld
通讯作者：
K. Kornfeld;M. Reitman;R. Kornfeld