The function and mechanism of COX-2 in angiogenesis of gastric cancer cells.
The function and mechanism of COX-2 in angiogenesis of gastric cancer cells.
复制标题
COX-2在胃癌细胞血管生成中的作用及机制
DOI：
10.1186/1756-9966-30-13
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发表时间：
2011-01-25
期刊：
影响因子：
--
通讯作者：
Fan D
中科院分区：
文献类型：
--
作者：
Yao L;Liu F;Hong L;Sun L;Liang S;Wu K;Fan D
本研究旨在探讨考克斯-2 siRNA对胃癌细胞增殖和血管生成的影响。将考克斯-2 siRNA转染胃癌细胞株SGC 7901，通过体内外实验检测细胞生长和血管生成情况。采用人基因芯片、RT-PCR和western blot检测考克斯-2表达降低的细胞中血管生成相关分子的差异表达。下调考克斯-2的表达可显著抑制胃癌细胞在体内外的生长，抑制人脐静脉内皮细胞的迁移和管腔形成。共发现23种血管生成相关分子参与了考克斯-2诱导的血管生成抑制作用。RT-PCR和western blot结果显示，下调考克斯-2表达可抑制VEGF、Flt-1、Flk-1/KDR、Angiopoietin-1、tie-2、MMP 2和OPN的表达。考克斯-2可能介导肿瘤血管生成和生长，有望成为胃癌治疗的靶点。
Here we aimed to investigate the effect of COX-2 siRNA on proliferation and angiogenesis of gastric cancer cells. The gastric cancer cell line SGC7901 was transfected with COX-2 siRNA, then the growth and angiogenesis of cells were detected by in vitro and in vivo assay. Human microarray, RT-PCR and western blot were used to identify differentially expressed angiogenesis-related molecules in cells with decreased expression of COX-2. Down-regulation of COX-2 could significantly inhibit the in vitro and in vivo growth of gastric cancer cells, and suppress the migration and tube formation of human umbilical vein endothelial cells. Totally 23 angiogenesis-related molecules were found involved in COX-2-induced angiogenesis suppression. The results of RT-PCR and western blot showed that down-regulation of COX-2 might inhibit VEGF, Flt-1, Flk-1/KDR, angiopoietin-1, tie-2, MMP2 and OPN. COX-2 might mediate tumor angiogenesis and growth, and could be considered as a target for gastric cancer therapy.
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影响因子：
120.1
作者：
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通讯作者：
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影响因子：
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作者：
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影响因子：
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影响因子：
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