An assay for pattern formation in dictyostelium discoideum using monoclonal antibodies, flow cytometry, and subsequent data analysis.
An assay for pattern formation in dictyostelium discoideum using monoclonal antibodies, flow cytometry, and subsequent data analysis.
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使用单克隆抗体、流式细胞术和随后的数据分析对盘基网柄菌中的模式形成进行测定。
DOI：
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发表时间：
1984
期刊：
影响因子：
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通讯作者：
Keith L. Williams
中科院分区：
文献类型：
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作者：
Ludwig Voet;Marianne Krefft;Helga Mairhofer;Keith L. Williams
利用前孢子特异性单克隆抗体和荧光激活细胞分选仪，建立了一种测定盘基网柄藻蛞蝓阶段前孢子细胞比例的方法。开发了适当的数据分析技术。段塞大小和年龄的影响进行了测定。小蛞蝓比大蛞蝓的前孢子细胞百分比低。前孢子细胞的百分比增加，然后在几个小时和9天之间的蛞蝓年龄下降。观察到对老化蛞蝓细胞大小的显著影响。特别是未标记的（主要是prestalk）细胞比前孢子细胞在年轻的蛞蝓，但6天的迁移后，他们变得比前孢子细胞小得多。所有未标记细胞的大小都发生了协调的变化，这表明这些细胞（包括前茎细胞、前茎样细胞和前盘细胞）彼此相关。
An assay for determining the proportions of prespore cells in a simple multicellular organism, the slug stage of Dictyostelium discoideum, was established using a prespore-specific monoclonal antibody and a fluorescence-activated cell sorter. Appropriate techniques for data analysis were developed. The effects of slug size and age were determined. Small slugs have a lower percentage of prespore cells than large slugs. The percentage of prespore cells increases and then decreases in slugs aged between a few hours and 9 days. Pronounced effects were observed on the size of cells in aging slugs. In particular unlabelled (mostly prestalk) cells were larger than prespore cells in young slugs, but after 6 days migration they became considerably smaller than prespore cells. The fact that all unlabelled cells were coordinately shifted in size, suggests that these cells (which comprise prestalk, prestalklike, and predisc cells) are related to each other.
使用单克隆抗体检测盘基网柄菌和粘液盘基网柄菌的前孢子细胞之间的抗原差异。
影响因子：
3.7
作者：
Gregg,JH;Krefft,M;Haas-Kraus,A;Williams,KL
通讯作者：
Williams,KL