Skin tape stripping identifies gene transcript signature associated with allergic contact dermatitis.
Skin tape stripping identifies gene transcript signature associated with allergic contact dermatitis.
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DOI：
10.1111/cod.13749
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发表时间：
2021-05
影响因子：
5.5
通讯作者：
Yu J
中科院分区：
文献类型：
--
作者：
Tam I;Hill KR;Park JM;Yu J
过敏性接触性皮炎（ACD）和刺激性接触性皮炎（ICD）是常见的皮肤病，临床和组织学表现重叠，但不同的潜在机制。斑贴试验是ACD诊断的金标准，但其结果的解释可能会受到弱和变化的宏观反应的混淆。检查通过胶带剥离（TS）从斑贴试验患者皮肤取样的RNA的基因转录谱是否可以区分ACD与ICD和基线皮肤状态（对照; CON）。招募了9名通过斑贴试验确诊为ACD的患者（7名女性和2名男性;年龄24-72岁[平均38.6岁]）。从TS样品中分离总RNA，并使用39种基因特异性引物通过定量实时PCR测定相对转录物丰度。TS捕获来自不同皮肤细胞类型的基因转录本，不仅包括角质形成细胞，还包括表皮和真皮抗原呈递细胞。在转录谱分析中分析的基因中，编码表皮屏障组分和炎症介质的基因与ICD或CON皮肤相比在ACD皮肤中表现出转录丰度的变化。我们的研究结果揭示了在斑贴试验和基于分子标记的ACD诊断过程中，皮肤TS用于非侵入性活检的潜力。
Allergic contact dermatitis (ACD) and irritant contact dermatitis (ICD) are common skin conditions with overlapping clinical and histologic appearance but distinct underlying mechanisms. Patch testing is the gold standard for ACD diagnosis, yet the interpretation of its results may be confounded by weak and varying macroscopic reactions. To examine whether gene transcript profiling of RNA sampled from patch-tested patient skin by tape stripping (TS) could differentiate ACD from ICD and the baseline skin state (control; CON). Nine patients (7 females and 2 males; age 24–72 [mean 38.6]) with confirmed ACD through patch testing were recruited. Total RNA was isolated from TS samples and relative transcript abundance was determined by quantitative real-time PCR using 39 gene-specific primers. TS captured gene transcripts derived from diverse skin cell types including not only keratinocytes but also epidermal and dermal antigen-presenting cells. Among the genes analysed in transcript profiling, genes encoding epidermal barrier components and inflammatory mediators exhibited changes in transcript abundance in ACD skin compared to ICD or CON skin. Our findings reveal the potential of skin TS for non-invasive biopsy during patch testing and molecular marker-based ACD diagnosis.
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影响因子：
17.1
作者：
Leung DYM;Calatroni A;Zaramela LS;LeBeau PK;Dyjack N;Brar K;David G;Johnson K;Leung S;Ramirez-Gama M;Liang B;Rios C;Montgomery MT;Richers BN;Hall CF;Norquest KA;Jung J;Bronova I;Kreimer S;Talbot CC Jr;Crumrine D;Cole RN;Elias P;Zengler K;Seibold MA;Berdyshev E;Goleva E
通讯作者：
Goleva E
DOI：
10.1016/j.jaci.2017.10.046
发表时间：
2018-04
期刊：
The Journal of allergy and clinical immunology
影响因子：
--
作者：
Dyjack N;Goleva E;Rios C;Kim BE;Bin L;Taylor P;Bronchick C;Hall CF;Richers BN;Seibold MA;Leung DYM
通讯作者：
Leung DYM
影响因子：
10.9
作者：
Guttman-Yassky, Emma;Diaz, Aisleen;Paller, Amy S.
通讯作者：
Paller, Amy S.
影响因子：
14.9
作者：
Metsalu T;Vilo J
通讯作者：
Vilo J
影响因子：
13.8
作者：
Lim, Henry W.;Collins, Scott A. B.;Moyano, Jose V.
通讯作者：
Moyano, Jose V.