Fluorescence Modified Chitosan-Coated Magnetic Nanoparticles for High-Efficient Cellular Imaging.
Fluorescence Modified Chitosan-Coated Magnetic Nanoparticles for High-Efficient Cellular Imaging.
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DOI：
10.1007/s11671-008-9239-9
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发表时间：
2009-01-16
影响因子：
--
通讯作者：
Gu N
中科院分区：
文献类型：
--
作者：
Ge Y;Zhang Y;He S;Nie F;Teng G;Gu N
用纳米颗粒标记细胞用于活体检测是各种生物医学应用感兴趣的。在这项研究中，新的荧光/磁性纳米粒子的制备和用于高效的细胞成像。改性壳聚糖包覆的纳米颗粒具有磁性氧化物核和共价连接的荧光染料。我们评估了用纳米颗粒标记癌细胞（SMMC-7721）的可行性和效率。通过流式细胞术和磁共振成像证实了纳米颗粒对细胞具有高亲和力。结果表明，纳米颗粒的细胞标记效率与孵育时间和纳米颗粒的浓度有关。用临床用1.5-T磁共振成像仪检测到的标记细胞数最少为104个。荧光和透射电子显微镜仪器被用来监测磁性纳米颗粒在细胞中的定位模式。这些新的磁荧光纳米剂已经证明了未来医疗用途的潜力。
Labeling of cells with nanoparticles for living detection is of interest to various biomedical applications. In this study, novel fluorescent/magnetic nanoparticles were prepared and used in high-efficient cellular imaging. The nanoparticles coated with the modified chitosan possessed a magnetic oxide core and a covalently attached fluorescent dye. We evaluated the feasibility and efficiency in labeling cancer cells (SMMC-7721) with the nanoparticles. The nanoparticles exhibited a high affinity to cells, which was demonstrated by flow cytometry and magnetic resonance imaging. The results showed that cell-labeling efficiency of the nanoparticles was dependent on the incubation time and nanoparticles’ concentration. The minimum detected number of labeled cells was around 104by using a clinical 1.5-T MRI imager. Fluorescence and transmission electron microscopy instruments were used to monitor the localization patterns of the magnetic nanoparticles in cells. These new magneto-fluorescent nanoagents have demonstrated the potential for future medical use.
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