Chromatin restoration following nucleotide excision repair involves the incorporation of ubiquitinated H2A at damaged genomic sites.
Chromatin restoration following nucleotide excision repair involves the incorporation of ubiquitinated H2A at damaged genomic sites.
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DOI：
10.1016/j.dnarep.2008.11.007
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发表时间：
2009-02-01
期刊：
影响因子：
3.8
通讯作者：
Wani, Altaf A.
中科院分区：
文献类型：
--
作者：
Zhu, Qianzheng;Wani, Gulzar;Arab, Hany H.;Ei-Mahdy, Mohamed A.;Ray, Alo;Wani, Altaf A.
在DNA损伤处理后恢复功能完整的染色质结构对于维持人类细胞的遗传和表观遗传信息至关重要。在这里，我们展示了在缺乏XPC、DDB2CSA或CSB的细胞中紫外线诱导的uH2A焦点的形成，但在缺乏XPA、XPG或XPF的细胞中不能诱导uH2A焦点的形成，这表明uH2A的掺入依赖于通过GGR或TCR子途径发生的成功的损伤修复。相反，在异步化细胞中形成γH_2AX焦点不需要X-PA、X-PG或X-PF。值得注意的是，多梳抑制物复合体1的成分H_2A泛素连接酶Ring1b没有定位于DNA损伤部位。然而，组蛋白伴侣CAF-1对损伤部位有明显的定位。CAF-1 p60基因敲除后，CAF-1和uH_2A焦点的形成均消失。染色质中CAF-1P150与NER因子TFIIH、RPA p70和增殖细胞核抗原相关。这些数据表明，基因组损伤的成功的NER和CAF-1介导的染色质修复迅速在染色质内的损伤修复部位连接了uH2A的掺入。
Restoration of functionally intact chromatin structure following DNA damage processing is crucial for maintaining genetic and epigenetic information in human cells. Here, we show the UV-induced uH2A foci formation in cells lacking XPC, DDB2, CSA or CSB, but not in cells lacking XPA, XPG or XPF indicating that uH2A incorporation relied on successful damage repair occurring through either GGR or TCR sub-pathway. In contrast, XPA, XPG or XPF were not required for formation of γH2AXfoci in asynchronous cells. Notably, the H2A ubiquitin ligase Ring1B, a component of Polycomb repressor complex 1, did not localize at DNA damage sites. However, histone chaperone CAF-1 showed distinct localization to the damage sites. Knockdown of CAF-1 p60 abolished CAF-1 as well as uH2A foci formation. CAF-1 p150 was found to associate with NER factors TFIIH, RPA p70 and PCNA in chromatin. These data demonstrate that successful NER of genomic lesions and prompt CAF-1-mediated chromatin restoration link uH2A incorporation at the sites of damage repair within chromatin.
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影响因子：
4.5
作者：
Houlard, Martin;Berlivet, Soizik;Probst, Aline V.;Quivy, Jean-Pierre;Hery, Patrick;Almouzni, Genevieve;Gerard, Matthieu
通讯作者：
Gerard, Matthieu
影响因子：
56.9
作者：
Brueckner, Florian;Hennecke, Ulrich;Cramer, Patrick
通讯作者：
Cramer, Patrick
影响因子：
56.9
作者：
CHU, G;CHANG, E
通讯作者：
CHANG, E
影响因子：
11.2
作者：
Li, Jinyou;Wang, Qi-En;Wani, Altaf A.
通讯作者：
Wani, Altaf A.
DOI：
10.1073/pnas.1635158100
发表时间：
2003-10-14
影响因子：
11.1
作者：
Hoek, M;Stillman, B
通讯作者：
Stillman, B