Betulinic acid augments the inhibitory effects of vincristine on growth and lung metastasis of B16F10 melanoma cells in mice.
Betulinic acid augments the inhibitory effects of vincristine on growth and lung metastasis of B16F10 melanoma cells in mice.
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DOI：
10.1038/sj.bjc.6601746
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发表时间：
2004-04-19
影响因子：
8.8
通讯作者：
中科院分区：
文献类型：
--
作者：
本文研究了白桦酸(BA)和长春新碱(VCR)联用对小鼠黑色素瘤B16F10细胞的体内外抗肿瘤作用。白桦酸是一种五环三萜类化合物，联合应用VCR对黑色素瘤细胞有协同杀伤作用。白桦酸和长春新碱可诱导B16F10黑色素瘤细胞发生不同程度的细胞周期停滞(BA停滞于G1期，VCR停滞于G2/M期)，并诱导细胞发生凋亡。在体内研究中，VCR抑制了肿瘤细胞向肺部的转移。VCR中加入BA可增强对C57BL/6小鼠黑色素瘤细胞实验性肺转移的抑制作用。BA联合长春新碱治疗组小鼠肺内直径大于1 mm的肺结节数明显少于长春新碱对照组。提示BA是提高恶性黑色素瘤化疗疗效的有效补充剂。
We examined the antitumour effect of a combination of betulinic acid (BA) and vincristine (VCR) on murine melanoma B16F10 cells in vitro and in vivo. Betulinic acid, a pentacyclic triterpene, showed a synergistic cytotoxic effect on melanoma cells by combinational use of VCR. Betulinic acid and VCR induced cell cycle arrest at different points (BA at G1 phase and VCR at G2/M phase) and caused apoptosis in B16F10 melanoma cells. In the in vivo study, VCR inhibited metastasis of tumour cells to the lung. The addition of BA to VCR augmented suppression of the experimental lung metastasis of melanoma cells in C57BL/6 mice. The number of lung nodules of more than 1 mm in diameter in mice treated with BA and VCR was less than that in mice treated with VCR alone. These results suggest that BA is an effective supplement for enhancing the chemotherapeutic effect on malignant melanoma.
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影响因子：
8.4
作者：
Kivelä, T;Suciu, S;Pyrhönen, S
通讯作者：
Pyrhönen, S
影响因子：
5.1
作者：
Ko, HH;Yu, SM;Lin, CN
通讯作者：
Lin, CN
影响因子：
4
作者：
DARZYNKIEWICZ, Z
通讯作者：
DARZYNKIEWICZ, Z
影响因子：
4.8
作者：
Lee, Y;Shacter, E
通讯作者：
Shacter, E
DOI：
10.1111/j.1349-7006.2002.tb01273.x
发表时间：
2002-04
期刊：
Japanese journal of cancer research : Gann
影响因子：
--
作者：
Kwon HJ;Shim JS;Kim JH;Cho HY;Yum YN;Kim SH;Yu J
通讯作者：
Yu J