Monitoring real-time enzymatic hydrolysis of Distillers Dried Grains with Solubles (DDGS) by dielectric spectroscopy following hydrothermal pre-treatment by steam explosion.
Monitoring real-time enzymatic hydrolysis of Distillers Dried Grains with Solubles (DDGS) by dielectric spectroscopy following hydrothermal pre-treatment by steam explosion.
复制标题
在蒸汽爆炸水热预处理后，通过介电谱监测含可溶物干酒糟 (DDGS) 的实时酶水解。
DOI：
10.1016/j.biortech.2012.09.021
复制
发表时间：
2013
影响因子：
11.4
通讯作者：
Bryant DN
中科院分区：
文献类型：
--
作者：
Bryant DN
介电谱（DS）已被用于监测同步糖化和发酵的木质纤维素生物质通过测量其介电状态。然而，这是未知的蒸汽爆破（SE）预处理后，木质纤维素是否仍将保持介电状态，如果保持，是否可以监测酶水解过程中的耗散。经SE预处理的干酒糟（Distillers Dried Grains with Solubles，DDGS）的电容（C= 580 kHz）约为24 pFcm −1。在加入全强度纤维素分解混合物A（CC-A; R2=0.97）和1/3强度混合物B（CC-B; R2=0.96）后，测定电容的自然对数衰减。此外，DS生物质探针定量了水解过程中电容耗散的初始线性速率。CC-B组的发生率为CC-A组的34%。这些数据扩展了DS生物质探针用于实时监测SE预处理的木质纤维素底物的酶水解的范围和实用性。
Dielectric spectroscopy (DS) has been used to monitor the simultaneous saccharification and fermentation of lignocellulosic biomass by measuring its dielectric state. However, it is unknown whether following steam explosion (SE) pre-treatment, lignocellulose would still maintain a dielectric state, and, if maintained, whether the dissipation during enzymatic hydrolysis could be monitored. Distillers Dried Grains with Solubles (DDGS), pre-treated by SE, was found to have a capacitance (C=580kHz) of approximately 24pFcm−1. Following addition of full-strength cellulolytic cocktail A (CC-A; R2=0.97) and 1/3 strength cocktail B (CC-B; R2=0.96), a natural logarithmic decay in capacitance was determined. Furthermore, the DS biomass probes quantified the initial linear rate of dissipation in capacitance during hydrolysis. The rate of CC-B was 34% that of CC-A. These data extend scope and utility of DS biomass probes for monitoring the enzymatic hydrolysis of SE-pre-treated lignocellulosic substrates in real-time.
影响因子：
11.4
作者：
Bryant, David N.;Morris, Stephen M.;Gallagher, Joe A.
通讯作者：
Gallagher, Joe A.