Glomerular handling of immune complex in the acute phase of active in situ immune complex glomerulonephritis employing cationized ferritin in rats
Glomerular handling of immune complex in the acute phase of active in situ immune complex glomerulonephritis employing cationized ferritin in rats
复制标题
使用阳离子化铁蛋白在大鼠活性原位免疫复合物肾小球肾炎急性期中对免疫复合物的肾小球处理
作者：
Y. Fujigaki;M. Nagase;K. Kojima;Tatsuo Yamamoto;A. Hishida
摘要用阳离子铁蛋白对活动性原位肾炎大鼠肾小球免疫复合物(IC)和炎症介质系统的超微结构定位进行了 观察。采用单侧肾灌注阳离子铁蛋白作为抗原(Ag)的方法诱导大鼠肾小球肾炎，并用免疫金电子显微镜对抗体、C3和大鼠C5b-9复合体进行定位。银染抗体复合物最初形成于内皮下，与补体C3结合，可吸引血小板、中性粒细胞和单核细胞。1天后，不含C3的Ag-Ab复合体穿过肾小球基底膜，在肾小球基底膜下重新聚集，并伴有C3沉积。不含C3的Ag-Ab复合体在1d内在足细胞间隙聚集，6h在上皮细胞内可见C5b-9复合体，C5b-9复合体存在于上皮下免疫沉积和膜泡中，而在内皮下免疫沉积中仅有极少量。聚集的血小板、中性粒细胞和单核细胞与内皮细胞或内皮下IC直接接触。中性粒细胞和单核细胞胞浆内空泡中含有抗原、抗体和C3。2 h后，在与内皮下区相邻的系膜基质中也可见Ag-Ab复合体，且数量略有增加，此后系膜区扩张。大多数在内皮下间隙形成的IC迅速形成大小为激活C3的晶格，然后被转移到上皮下间隙。C3在内皮下区溶解ICs的潜在能力在这一过程中可能是重要的。中性粒细胞和/或单核细胞对内皮下ICs的吞噬作用以及ICs向系膜基质的移动也可能有助于IC从内皮下间隙中去除。
Abstract The ultrastructural localization of immune complex (IC) and inflammatory mediator systems in the glomerulus was investigated in active in situ IC glomerulonephritis employing cationized ferritin in rats. Glomerulonephritis was induced by unilateral renal perfusion of cationized ferritin as antigen (Ag) in preimmunized rats, and anti-ferritin antibody (Ab), C3 and the rat C5b–9 complex were localized by means of immunogold electron microscopy. Ag–Ab complexes were initially formed subendothelially, associated with C3, and attracted platelets, polymorphonuclear leucocytes (PMN) and monocytes. Then Ag–Ab complexes, without C3, passed across the glomerular basement membrane to re-aggregate subepithelially accompanied by C3 deposition after 1 day. Ag–Ab complexes without C3 accumulated in the inter-podocyte space within 1 day and were seen in the epithelial cells at 6 h. C5b–9 complexes were found in subepithelial immune deposits and in membrane vesicles of the epithelial cells, but only in very small amounts in subendothelial immune deposits. Accumulated platelets, PMN, and monocyte were in direct contact with endothelial cells or subendothelial IC. PMN and monocytes contained Ag, Ab and C3 in intracytoplasmic vacuoles. Ag–Ab complexes were also found in the mesangial matrix adjacent to the subendothelial region after 2 h and increased slightly in number, with expansion of the mesangial area thereafter. Most ICs formed in the subendothelial space rapidly formed lattices of a size that activated C3 and were then translocated to the subepithelial space. The potential ability of C3 to solubilize ICs in the subendothelial region may be important in this process. Endocytosis of subendothelial ICs by PMN and/or monocytes and the movement of ICs to the mesangial matrix may also contribute to the removal of IC from the subendothelial space.
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影响因子：
19.6
作者：
Makino,H;Lelongt,B;Kanwar,YS
通讯作者：
Kanwar,YS
免疫复合物诱导血小板活化后血小板阳离子蛋白的肾小球定位。
DOI：
--
发表时间：
1990
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
Barnes,Jl;Camussi,G;Tetta,C;Venkatachalam,MA
通讯作者：
Venkatachalam,MA
免疫复合物诱导的人单核细胞促凝血活性。
DOI：
--
发表时间：
1982
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Schwartz,BS;Levy,GA;Edgington,TS
通讯作者：
Edgington,TS
影响因子：
4.4
作者：
D. Kerjaschki;M. Schulze;S. Binder;Renate Kain;P. Ojha;M. Susani;Reinhard Horvat;P. Baker;W. Couser
通讯作者：
D. Kerjaschki;M. Schulze;S. Binder;Renate Kain;P. Ojha;M. Susani;Reinhard Horvat;P. Baker;W. Couser
C5b-9 尿排泄增加可区分大鼠被动海曼肾炎。
影响因子：
19.6
作者：
Schulze,M;Baker,PJ;Perkinson,DT;Johnson,RJ;Ochi,RF;Stahl,RA;Couser,WG
通讯作者：
Couser,WG