FLAVONOIDS AND PHENOLIC CONTENT IN WHEAT GRASS PLANT (TRITICUM AESTIVUM).
FLAVONOIDS AND PHENOLIC CONTENT IN WHEAT GRASS PLANT (TRITICUM AESTIVUM).
复制标题
小麦草植物（TRITICUM AESTIVUM）中的类黄酮和酚类含量。
DOI：
10.1016/b978-0-12-078148-5.50013-0
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
S. Malla
中科院分区：
文献类型：
--
作者：
Seyed Hossein Zendehbad;Mohammad Javad Mehran;S. Malla
本研究旨在筛选小麦草（Triticum aestivum）叶片的各种溶剂提取物，以显示其有效的抗氧化活性，为寻找新型抗氧化剂在食品和药物补充剂中的可能来源。研究了黄芪甲醇提取物和氯仿提取物的抗氧化活性。首先对各组分进行酚类和类黄酮的筛选，然后对总多酚类和类黄酮的含量进行估算。此外，该馏分用于显示潜在的抗氧化活性。通过对2,2-二苯基-1-苦味酰肼（DPPH）自由基的清除活性和对H2O2的清除活性来证明其抗氧化活性。该植物对筛选的两种植物化学物质均有阳性反应。甲醇馏分的总酚含量为44%，氯仿馏分的总酚含量为12%。氯仿部位总黄酮含量（40.420±0.13 μg/mL）高于甲醇部位（22.873±0.05）。甲醇馏分含有更多的多酚（44%），比甲醇馏分（12%）。甲醇组分具有较强的DPPH清除活性（IC50为4.258±0.54 min），而阳性对照抗坏血酸为3.3266±0.38 min。氯仿部位的清除活性为（IC50 5.217±0.52 min）。小麦草各溶剂提取物对过氧化氢的清除活性依次为阳性对照bb0甲醇bb1氯仿。H2O2活性数据显示，活性呈剂量依赖性。在浓度为200 μg/mL时，氯仿、甲醇和阳性对照的H2O2活性分别为57.11%、71.13%和86.54%。关键词：小麦，总酚类物质，2,2-二苯基-1-苦基肼基，抗氧化，H2O2清除活性
The study was mainly designed to screen various solvent extracts of the leaves of wheatgrass (Triticum aestivum) to show the potent antioxidant activity in order to find possible sources for novel antioxidants in food and pharmaceutical supplements. A detailed study was performed on the antioxidant activity of the methanol extract and chloroform extract of T. aestivum. The fractions were initially screened for the phenolics and flavonoids and then estimated for the total polyphenols and the flavonoids. Further, the fractions were used for showing the potential antioxidant activity. The antioxidant activity was displayed by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity and H2O2 scavenging activity. The plant responded positive for both the phytochemicals screened. The total phenolic content was found to be more for methanol fraction (44%) when compared with the chloroform fraction (12%). The total flavonoids were more for the chloroform fraction (40.420 ± 0.13 μg/mL) when compared with the methanol fraction (22.873 ± 0.05). Methanol fraction was shown to contain more polyphenols (44%), than the methanol fraction (12%). Methanol fraction displayed strong DPPH scavenging activity (IC50 4.258 ± 0.54 minute) in reference to the positive control ascorbic acid exhibited 3.3266 ± 0.38 minute. The scavenging activities of the chloroform fraction were (IC50 5.217 ± 0.52 minute). The scavenging activity for hydrogen peroxide of various solvent extracts from Wheatgrass was in the order of positive control > methanol > chloroform respectively. The data from H2O2 activity showed that the activity was dose-dependent. The H2O2 activity at a concentration of 200 μg/mL was found to 57.11%, 71.13%, and 86.54% for the chloroform, methanol, and positive control fractions, respectively. Keywords: Triticum aestivum, Total phenolics, 2,2-diphenyl-1-picrylhydrazyl, Antioxidant, H2O2 scavenging activity.
影响因子：
56.9
作者：
AMES, BN
通讯作者：
AMES, BN