Comparative expression analysis of POU4F1, POU4F2 and ISL1 in developing mouse cochleovestibular ganglion neurons.
Comparative expression analysis of POU4F1, POU4F2 and ISL1 in developing mouse cochleovestibular ganglion neurons.
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POU4F1，POU4F2和ISL1的比较表达分析在发育小鼠耳目膜神经节神经元中。
DOI：
10.1016/j.gep.2014.03.001
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发表时间：
2014-05
影响因子：
1.2
通讯作者：
Gan, Lin
中科院分区：
文献类型：
--
作者：
Deng, Min;Yang, Hua;Xie, Xiaoling;Liang, Guoqing;Gan, Lin
POU-同源结构域和LIM-同源结构域转录因子在视网膜、内耳、背根神经节和三叉神经节内发育中的投射神经元中表达，并且在它们的分化和存活中发挥协同作用。在这里，使用免疫组织化学，我们提出了一个比较分析的时空表达模式POU4F1，POU4F2和ISL 1在小鼠内耳的耳蜗前庭神经节（CVG）神经元的发展。在早期阶段，当耳神经元首先在耳上皮（OE）中检测到并迁移到耳周间充质中以形成CVG时，POU4F1和ISL 1在大多数分层的CVG神经元中共表达，所述分层的CVG神经元由NEUROD 1表达标记，但POU4F1在耳上皮中不存在。POU4F2的表达开始于POU4F1和ISL 1的表达之后，并且在NEUROD1阴性的有丝分裂后CVG神经元中观察到。当CVG神经元支配前庭和耳蜗感觉器官时，POU4F1、POU4F2和ISL 1的表达在前庭和螺旋神经节细胞中继续。在发育后期，POU4F1表达在大多数螺旋神经节（SG）神经元中下调，更多的神经元表达POU4F2表达，而ISL 1表达保持不变。POU4F1、POU4F2和ISL1的差异表达和重叠表达结合以往的研究表明，这些转录因子在内耳神经元的发育中可能存在功能相互作用和调控关系。
POU-homeodomain and LIM-homeodomain transcription factors are expressed in developing projection neurons within retina, inner ear, dorsal root ganglion, and trigeminal ganglion, and play synergistic roles in their differentiation and survival. Here, using immunohistochemistry, we present a comparative analysis of the spatiotemporal expression pattern of POU4F1, POU4F2, and ISL1 during the development of cochleovestibular ganglion (CVG) neurons in mouse inner ear. At early stages, when otic neurons are first detected in the otic epithelium (OE) and migrate into periotic mesenchyme to form the CVG, POU4F1 and ISL1 are co-expressed in a majority of the delaminated CVG neurons, which are marked by NEUROD1 expression, but POU4F1 is absent in the otic epithelium. The onset of POU4F2 expression starts after that of POU4F1 and ISL1, and is observed in the NEUROD1-negative, post-mitotic CVG neurons. When the CVG neurons innervate the vestibular and cochlear sensory organs, the expression of POU4F1, POU4F2, and ISL1 continues in both vestibular and spiral ganglion cells. Later in development, POU4F1 expression becomes down-regulated in a majority of spiral ganglion (SG) neurons and more neurons express POU4F2 expression while ISL1 expression is maintained. The differential as well as overlapping expression of POU4F1, POU4F2, and ISL1 combined with previous studies suggests possible functional interaction and regulatory relationship of these transcription factors in the development of inner ear neurons.
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影响因子：
16.2
作者：
Karsten, Stanislav L.;Sang, Tzu-Kang;Jackson, George R.
通讯作者：
Jackson, George R.
影响因子：
2.7
作者：
Gan, L;Wang, SW;Klein, WH
通讯作者：
Klein, WH
影响因子：
2.5
作者：
ANNIKO, M;WIKSTROM, SO
通讯作者：
WIKSTROM, SO
DOI：
10.1523/jneurosci.0901-11.2011
发表时间：
2011-07-06
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Dykes IM;Tempest L;Lee SI;Turner EE
通讯作者：
Turner EE
DOI：
10.1002/neu.10098
发表时间：
2002-11-05
期刊：
JOURNAL OF NEUROBIOLOGY
影响因子：
--
作者：
Fritzsch, B;Beisel, KW;Reichardt, LF
通讯作者：
Reichardt, LF