Combined experimental and mathematical approach for development of microfabrication-based cancer migration assay.
Combined experimental and mathematical approach for development of microfabrication-based cancer migration assay.
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DOI：
10.1007/s10439-011-0337-y
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发表时间：
2011-09
影响因子：
3.8
通讯作者：
Baskaran, Harihara
中科院分区：
文献类型：
--
作者：
Sarkar, Saheli;Bustard, Bethany L.;Welter, Jean F.;Baskaran, Harihara
关键词：
癌细胞的迁移是转移的关键决定因素，其与患者的不良预后相关。有证据表明，癌细胞运动性是由基质细胞相互作用调节的。为了量化同型和异型细胞-细胞相互作用对迁移的作用，已经通过微加工技术开发了二维迁移测定。两种乳腺癌细胞系MDA-MB-231和MDA-MB-453用于开发癌细胞（细胞岛）的微图案，其在该测定中显示不同的迁移谱。尽管这些细胞的个体迁移率仅显示出7倍的差异，但MDA-MB-453岛的迁移显著低于MDA-MB-231岛，表明分离细胞与岛的迁移调节差异。岛的大小对迁移的影响最大，主要是MDA-MB-231细胞。MDA-MB-231岛的迁移通过与同型细胞的相互作用而减少，并且通过异型非癌相关成纤维细胞而显著更多。此外，还利用Stefan-Maxwell方程建立了多细胞群体中岛屿迁移的数学模型。该模型与实验结果定性一致，并预测细胞密度和岛的大小之间的双相关系。结合实验和数学模型可以用来定量研究细胞间相互作用对迁移的影响。
Migration of cancer cells is a key determinant of metastasis, which is correlated with poor prognosis in patients. Evidence shows that cancer cell motility is regulated by stromal cell interactions. To quantify the role of homotypic and heterotypic cell-cell interaction on migration, a two-dimensional migration assay has been developed by microfabrication techniques. Two breast cancer cell lines, MDA-MB-231 and MDA-MB-453, were used to develop micropatterns of cancer cells (cell islands) that revealed distinct migration profiles in this assay. Although the individual migration rates of these cells showed only a seven-fold difference, MDA-MB-453 islands migrated significantly lower than MDA-MB-231 islands, indicating differential regulation of migration in isolated cells vs. islands. Island size had the greatest impact on migration, primarily for MDA-MB-231 cells. Migration of MDA-MB-231 islands was decreased by interaction with homotypic cells, and significantly more by heterotypic non-cancer associated fibroblasts. In addition, a mathematical model of island migration in multi-cellular population has been developed using Stefan-Maxwell's equation. The model showed qualitative agreement with experimental results and predicted a biphasic relation between cell densities and island sizes. The combined experimental and mathematical model can be used to quantitatively study the impact of cell-cell interactions on migration.
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影响因子：
2
作者：
Kim, Yangjin;Lawler, Sean;Friedman, Avner
通讯作者：
Friedman, Avner
影响因子：
78.5
作者：
Klein, Christoph A.
通讯作者：
Klein, Christoph A.
DOI：
10.1124/jpet.105.093252
发表时间：
2005-12-01
影响因子：
3.5
作者：
Dollé, JP;Rezvan, A;Lelkes, PI
通讯作者：
Lelkes, PI
影响因子：
4.8
作者：
Neiva, Kathleen G.;Zhang, Zhaocheng;Nor, Jacques E.
通讯作者：
Nor, Jacques E.
DOI：
10.1093/imammb/dql007
发表时间：
2006-09-01
影响因子：
1.1
作者：
Marchant, Ben P.;Norbury, John;Byrne, Helen M.
通讯作者：
Byrne, Helen M.