The role of hemidesmosomes and focal contacts in the skin visualized by dual-color live cell imaging
The role of hemidesmosomes and focal contacts in the skin visualized by dual-color live cell imaging
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通过双色活细胞成像可视化半桥粒和皮肤焦点接触的作用
DOI：
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发表时间：
2014
影响因子：
1.8
通讯作者：
D. Tsuruta
中科院分区：
文献类型：
--
作者：
T. Ozawa;S. Hiroyasu;D. Tsuruta
活细胞成像是阐明蛋白质动力学的有力工具。我们的小组集中在两个主要的细胞-基质粘附装置，半桥粒和角化细胞中的焦点接触的动力学。首先，我们通过单色活细胞成像技术观察了角质形成细胞生理状态下半桥粒蛋白和局部接触蛋白的命运。半桥粒蛋白和焦点接触蛋白都是高度动态的。接下来，为了观察半桥粒蛋白和焦点接触蛋白之间的相互作用，我们通过双色活细胞成像同时观察这些蛋白在角质形成细胞的生理设置和创伤设置中的命运。这些半桥粒蛋白和焦点接触蛋白在生理环境中表现出最小重叠表达的个体动态。与此形成鲜明对比的是，这两种蛋白质在角质形成细胞的伤口愈合中表现出高度调节的相互作用。最后，我们观察了自身免疫性大疱病、大疱性类天疱疮的主要靶点、半桥粒的组成成分BP 180蛋白在抗BP 180自身抗体存在下的命运。结果，在这种情况下，BP 180分子被内化，因此角质形成细胞显示出对细胞基质的粘附减弱。我们的工作阐明了动态方面的细胞-基质粘附装置在生理和病理条件下。
Live cell imaging is a powerful tool to elucidate dynamics of protein(s). Our group has concentrated on dynamics of two major cell-matrix adhesion devices, hemidesmosome and focal contact in the keratinocytes. Firstly, we observed the fate of hemidesmosome protein or focal contact protein by single-color live cell imaging in the physiological setting of keratinocytes. Both hemidesmosome proteins and focal contact proteins were highly dynamic. Next, in order to observe the interaction between hemidesmosome protein and focal contact protein, we observed the fate of these proteins at the same time by dual-color live cell imaging in physiological setting and in wound setting of keratinocytes. These hemidesmosome proteins and focal contact proteins showed individual dynamics with minimal overlap expressions in physiological settings. In sharp contrast, both proteins showed highly regulated interaction in wound setting of keratinocytes. Finally, we observed the fate of BP180 protein, which is a major target of autoimmune bullous disease, bullous pemphigoid, and component of hemidesmosome, under the existence of anti-BP180 autoantibody. In results, under such a circumstance, BP180 molecules were internalized and thus keratinocyte showed weakened adhesion to the cell matrix. Our work has elucidated dynamic aspects of cell-matrix adhesion devices under both physiological and pathological conditions.
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影响因子：
--
作者：
Tsuruta, D;Hopkinson, SB;Jones, JCR
通讯作者：
Jones, JCR
影响因子：
15.9
作者：
Liu, Z;Giudice, GJ;Diaz, LA
通讯作者：
Diaz, LA
影响因子：
15.9
作者：
Nelson, Kelly C.;Zhao, Minglang;Liu, Zhi
通讯作者：
Liu, Zhi
影响因子：
4.4
作者：
Ramzy S. Labib;G. Anhalt;Harikrishna Patel;D. Mutasim;L. Diaz
通讯作者：
Ramzy S. Labib;G. Anhalt;Harikrishna Patel;D. Mutasim;L. Diaz
影响因子：
15.9
作者：
DIAZ, LA;RATRIE, H;GIUDICE, GJ
通讯作者：
GIUDICE, GJ