Karyotypic stability of Fragaria (strawberry) species revealed by cross-species chromosome painting

Karyotypic stability of Fragaria (strawberry) species revealed by cross-species chromosome painting
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通过跨物种染色体涂色揭示草莓物种的核型稳定性

DOI:
10.1007/s10577-021-09666-1
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发表时间:
2021-06
影响因子:
2.6
通讯作者:
Yonghua Han
Yonghua Han
中科院分区:
生物学2区
文献类型:
--
作者:
Manman Qu;Luyue Zhang;Kunpeng Li;Jianying Sun;Zongyun Li;Yonghua Han

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染色体核型分析在确定物种关系和多倍体物种起源方面特别有用。单个染色体的鉴定是核型发育的基础。对于草莓属植物来说,个体染色体的确定非常困难,因为它们的大小和形状都很相似。在这里,我们使用在Fragaria Vesca开发的一组基于寡核苷酸的探针识别了11个不同倍性的代表性Fragaria物种的所有染色体。根据单独鉴定的染色体,建立了完整的分子细胞遗传学核型。此外,我们还利用寡核苷酸探针将5S和45S rDNA基因座定位于16个Fragaria种的特定染色体上。我们发现这些Fragaria物种保持着非常保守的核型。在这些物种的染色体中没有观察到细胞学水平上的染色体间结构重排。尽管核型稳定和相似,但在几个多倍体物种中观察到同源染色体之间寡核苷酸探针的信号强度存在差异。此外,多数种的45S和5S rDNA的分布模式也存在差异。这些数据为几个多倍体Fragaria物种的起源提供了新的见解。
Chromosome karyotyping analysis is particularly useful in determining species relationships and the origin of polyploid species. Identification of individual chromosomes is the foundation for karyotype development. For Fragaria (strawberry) species, definitive identification of the individual chromosomes is extremely difficult because of their small size and similar shape. Here, we identified all chromosomes for 11 representative Fragaria species with different ploidy using a set of oligonucleotide-based probes developed in Fragaria vesca. Comprehensive molecular cytogenetic karyotypes were established based on the individually identified chromosomes. In addition, we used oligo probes to assign the 5S and 45S rDNA loci to specific chromosomes in 16 Fragaria species. We found that these Fragaria species maintained a remarkably conserved karyotype. No inter-chromosomal structural rearrangements at the cytological level were observed in any of the chromosomes among these species. Despite karyotypic stability and similarity, variations in the signal intensity of oligo probes were observed among the homologous chromosomes in several polyploid species. Moreover, most Fragaria species also showed differences in the distribution patterns of 45S and 5S rDNA. These data provide new insights into the origins of several polyploid Fragaria species.
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发表时间: 2005-08
期刊: Hortscience
影响因子: 1.9
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发表时间: 2006-10
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DOI: --
发表时间: 1971
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DOI: 10.17660/actahortic.2009.842.1
发表时间: 2009-08
期刊: --
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