Gender Differences in the Allergic Response of Mice Neonatally Exposed to Environmental Tobacco Smoke
Gender Differences in the Allergic Response of Mice Neonatally Exposed to Environmental Tobacco Smoke
复制标题
暴露于环境烟草烟雾的新生小鼠过敏反应的性别差异
DOI：
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复制
发表时间：
2002
期刊：
影响因子：
--
通讯作者：
L. Gershwin
中科院分区：
文献类型：
--
作者：
B. Seymour;K. Friebertshauser;J. Peake;K. Pinkerton;R. Coffman;L. Gershwin
暴露于环境烟草烟雾（ETS）已被证明会增加过敏致敏性和反应性，并且有一些迹象表明，ETS对过敏反应的影响在男性和女性中是不同的。从新生儿期到成年期暴露于ETS后，对卵清蛋白（OVA）致敏的IgE反应是否存在性别差异尚待确定。为了解决这一问题，我们研究了从出生到成年在吸烟和不吸烟条件下饲养的BALB/c小鼠的卵细胞敏感性的性别差异。在6周龄时（第0天），所有小鼠均腹腔注射含氢氧化铝佐剂的卵细胞，然后在第14天至第80天期间，三次暴露于1%雾化的卵细胞，每次20分钟。女性血清中总IgE和OVA特异性IgE、IgG1含量显著高于男性（p<0.05）。此外，这些性反应以及嗜酸性粒细胞在暴露于ETS的小鼠中进一步增强。与环境空气相比，暴露于ETS的雌性小鼠肺中IgE阳性细胞数量明显增加，而雄性小鼠肺中IgE阳性细胞数量明显增加（p<0.05）。在ETS暴露后再次刺激肺匀浆后，Th2细胞因子（IL4、IL5、IL10和IL13）也有所升高。这些数据表明雌性动物明显比雄性动物更容易受到ETS对过敏反应的影响。
Exposure to environmental tobacco smoke (ETS) has been shown to increase allergic sensitization and reactivity and there has been some suggestion that the influence of ETS on the allergic response is dissimilar in males and females. It is to be determined whether gender differences exist in the IgE response to ovalbumin (OVA) sensitization following ETS exposure from the neonatal period through adulthood. To address this thesis, we examined gender differences in OVA sensitization of BALB/c mice housed from birth through adulthood under smoking and nonsmoking conditions. At 6 weeks of age (day 0) all mice were injected i.p. with OVA in aluminum hydroxide adjuvant followed by three 20 min exposures to 1% aerosolized OVA between day 14 and 80. There were significantly (p<0.05) more total and OVA specific IgE and IgG1 in the serum of females compared to males. Moreover, these sex responses, along with eosinophilia, were further enhanced in mice exposed to ETS. There were also significantly more IgE positive cells in the lungs of female, but not male, mice exposed to ETS compared with ambient air (p<0.05). There was also an elevation of Th2 cytokines (IL4, IL5, IL10, and IL13) after re-stimulation of lung homogenates following ETS exposure. These data demonstrate that female animals are significantly more susceptible than males to the influence of ETS on the allergic response.
DOI：
10.1164/ajrccm.163.2.2006009
发表时间：
2001-02-01
影响因子：
24.7
作者：
Gilliland, FD;Li, YF;Peters, JM
通讯作者：
Peters, JM
影响因子：
32.4
作者：
Beebe, AM;Mauze, S;Coffman, RL
通讯作者：
Coffman, RL