Monitoring peripheral nerve degeneration in ALS by label-free stimulated Raman scattering imaging.
Monitoring peripheral nerve degeneration in ALS by label-free stimulated Raman scattering imaging.
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DOI：
10.1038/ncomms13283
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发表时间：
2016-10-31
影响因子：
16.6
通讯作者：
Eggan, Kevin
中科院分区：
文献类型：
--
作者：
Tian, Feng;Yang, Wenlong;Mordes, Daniel A.;Wang, Jin-Yuan;Salameh, Johnny S.;Mok, Joanie;Chew, Jeannie;Sharma, Aarti;Leno-Duran, Ester;Suzuki-Uematsu, Satomi;Suzuki, Naoki;Han, Steve S.;Lu, Fa-Ke;Ji, Minbiao;Zhang, Rosanna;Liu, Yue;Strominger, Jack;Shneider, Neil A.;Petrucelli, Leonard;Xie, X. Sunney;Eggan, Kevin
如果能够更容易地观察到运动轴突变性，将有助于肌萎缩侧索硬化症（ALS）的研究和潜在的干预措施。在这里，我们表明，受激拉曼散射（SRS）显微镜可用于灵敏地监测周围神经变性ALS小鼠模型和ALS尸检材料。症状前SOD1小鼠模型的三维成像和基于相关性的算法的数据处理显示，外周神经的显著变性可以与肌肉去神经支配的最早可检测迹象同时检测到，并且在生理上可测量的运动功能下降之前。我们还发现，外周变性是FUS以及ALS的C9ORF72重复扩增模型中的早期事件，并且连续成像允许在活体动物中长期观察疾病进展和药物作用。我们的研究表明，SRS成像是测量疾病进展的敏感和定量手段，极大地促进了疾病机制和候选疗法的未来研究。目前缺乏敏感和无标记的成像方法来可视化神经变性。在这里，作者表明，受激拉曼散射（SRS）显微镜可用于监测周围神经变性的肌萎缩侧索硬化症（ALS）的小鼠模型和ALS患者的死后组织。
The study of amyotrophic lateral sclerosis (ALS) and potential interventions would be facilitated if motor axon degeneration could be more readily visualized. Here we demonstrate that stimulated Raman scattering (SRS) microscopy could be used to sensitively monitor peripheral nerve degeneration in ALS mouse models and ALS autopsy materials. Three-dimensional imaging of pre-symptomatic SOD1 mouse models and data processing by a correlation-based algorithm revealed that significant degeneration of peripheral nerves could be detected coincidentally with the earliest detectable signs of muscle denervation and preceded physiologically measurable motor function decline. We also found that peripheral degeneration was an early event in FUS as well as C9ORF72 repeat expansion models of ALS, and that serial imaging allowed long-term observation of disease progression and drug effects in living animals. Our study demonstrates that SRS imaging is a sensitive and quantitative means of measuring disease progression, greatly facilitating future studies of disease mechanisms and candidate therapeutics. Sensitive and label-free imaging methods to visualize nerve degeneration are currently lacking. Here authors show that stimulated Raman scattering (SRS) microscopy can be used to monitor peripheral nerve degeneration in mouse models of amyotrophic lateral sclerosis (ALS) and in postmortem tissue from ALS patients.
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DOI：
10.1038/nrneurol.2013.153
发表时间：
2013-09
期刊：
Nature reviews. Neurology
影响因子：
--
作者：
通讯作者：
--
影响因子：
25
作者：
Kang, Shin H.;Li, Ying;Fukaya, Masahiro;Lorenzini, Ileana;Cleveland, Don W.;Ostrow, Lyle W.;Rothstein, Jeffrey D.;Bergles, Dwight E.
通讯作者：
Bergles, Dwight E.
影响因子：
3.5
作者：
Imitola, Jaime;Cote, Daniel;Khoury, Samia J.
通讯作者：
Khoury, Samia J.
DOI：
10.1126/science.aaa9344
发表时间：
2015-06-05
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Chew J;Gendron TF;Prudencio M;Sasaguri H;Zhang YJ;Castanedes-Casey M;Lee CW;Jansen-West K;Kurti A;Murray ME;Bieniek KF;Bauer PO;Whitelaw EC;Rousseau L;Stankowski JN;Stetler C;Daughrity LM;Perkerson EA;Desaro P;Johnston A;Overstreet K;Edbauer D;Rademakers R;Boylan KB;Dickson DW;Fryer JD;Petrucelli L
通讯作者：
Petrucelli L
影响因子：
5.3
作者：
Gould, Thomas W.;Buss, Robert R.;Oppenheim, Ronald W.
通讯作者：
Oppenheim, Ronald W.