Spatiotemporal coordination of cell division and growth during organ morphogenesis.
Spatiotemporal coordination of cell division and growth during organ morphogenesis.
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DOI：
10.1371/journal.pbio.2005952
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发表时间：
2018-11
期刊：
影响因子：
9.8
通讯作者：
Coen E
中科院分区：
文献类型：
--
作者：
Fox S;Southam P;Pantin F;Kennaway R;Robinson S;Castorina G;Sánchez-Corrales YE;Sablowski R;Chan J;Grieneisen V;Marée AFM;Bangham JA;Coen E
发育中的植物器官表现出复杂的生长、细胞分裂、细胞大小、细胞形状和器官形状的时空模式。解释这些模式是一个挑战，因为它们的动态和相互关联，这可能使人们难以从结果中分离出原因。为了解决这些问题，我们使用实时成像来确定不同遗传和组织背景下叶生长和分裂的时空模式。在无语（spch）突变体，它缺乏气孔谱系的简化背景下，表皮细胞层表现出明确的模式分裂，细胞大小，细胞形状，和生长沿着proximodistal和mediolateral轴。的模式和相关性是不同的，从那些观察到的连接subepidermal层，也不同于野生型的表皮层。通过计算模型，我们表明，结果可以解释为一个双重控制模型，时空控制操作的增长和细胞分裂，它们之间的交叉连接。由此产生的生长和分裂模式之间的相互作用导致变形叶内的细胞大小和形状的动态分布。通过调节模型的参数，我们说明了表型与细胞大小，细胞数量和器官大小的相关变化可能会产生。因此，该模型提供了一个综合的增长和分裂，可以作为进一步的实验研究的框架。器官形态发生涉及两个协调的过程：组织的生长和通过细胞分裂增加细胞数量。这两个过程已在许多系统中单独分析，并显示出在空间和时间上的复杂模式。然而，目前尚不清楚这些生长和细胞分裂模式在正在经历形状变化的生长叶子中是如何协调的。我们已经解决了这个问题，使用实时成像跟踪生长和细胞分裂的芥菜植物拟南芥的叶片。使用随后的计算建模，我们提出了一个综合模型的叶片生长和细胞分裂，产生动态分布的细胞大小和形状在不同的组织层，密切匹配实验观察到的。该模型的一个关键方面是生长和细胞分裂参数的时空模式的双重控制。通过调节模型中的参数，我们说明了表型如何与细胞大小，细胞数量和器官大小的变化相关。
A developing plant organ exhibits complex spatiotemporal patterns of growth, cell division, cell size, cell shape, and organ shape. Explaining these patterns presents a challenge because of their dynamics and cross-correlations, which can make it difficult to disentangle causes from effects. To address these problems, we used live imaging to determine the spatiotemporal patterns of leaf growth and division in different genetic and tissue contexts. In the simplifying background of the speechless (spch) mutant, which lacks stomatal lineages, the epidermal cell layer exhibits defined patterns of division, cell size, cell shape, and growth along the proximodistal and mediolateral axes. The patterns and correlations are distinctive from those observed in the connected subepidermal layer and also different from the epidermal layer of wild type. Through computational modelling we show that the results can be accounted for by a dual control model in which spatiotemporal control operates on both growth and cell division, with cross-connections between them. The interactions between resulting growth and division patterns lead to a dynamic distributions of cell sizes and shapes within a deforming leaf. By modulating parameters of the model, we illustrate how phenotypes with correlated changes in cell size, cell number, and organ size may be generated. The model thus provides an integrated view of growth and division that can act as a framework for further experimental study. Organ morphogenesis involves two coordinated processes: growth of tissue and increase in cell number through cell division. Both processes have been analysed individually in many systems and shown to exhibit complex patterns in space and time. However, it is unclear how these patterns of growth and cell division are coordinated in a growing leaf that is undergoing shape changes. We have addressed this problem using live imaging to track growth and cell division in the developing leaf of the mustard plant Arabidopsis thaliana. Using subsequent computational modelling, we propose an integrated model of leaf growth and cell division, which generates dynamic distributions of cell size and shape in different tissue layers, closely matching those observed experimentally. A key aspect of the model is dual control of spatiotemporal patterns of growth and cell division parameters. By modulating parameters in the model, we illustrate how phenotypes may correlate with changes in cell size, cell number, and organ size.
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影响因子：
10.5
作者：
Carabelli, Monica;Possenti, Marco;Ruberti, Ida
通讯作者：
Ruberti, Ida
影响因子：
7.4
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影响因子：
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作者：
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