Donor age and cell passage affects differentiation potential of murine bone marrow-derived stem cells.
Donor age and cell passage affects differentiation potential of murine bone marrow-derived stem cells.
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DOI：
10.1186/1471-2121-9-60
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发表时间：
2008-10-28
期刊：
影响因子：
--
通讯作者：
Cao Y
中科院分区：
文献类型：
--
作者：
Kretlow JD;Jin YQ;Liu W;Zhang WJ;Hong TH;Zhou G;Baggett LS;Mikos AG;Cao Y
骨髓间充质干细胞（BMSCs）是一种被广泛研究的成体干细胞群体，能够分化成各种谱系。由于组织工程的许多有前途的应用需要在收获后进行细胞扩增，并且涉及到老年人群中发现的疾病和病症的治疗，因此必须了解供体年龄和离体处理的影响，以便基于这些细胞开发临床技术和治疗方法。此外，目前对这两个因素如何相互影响知之甚少。观察了从不同年龄和不同传代次数的供体动物中获取的小鼠MSCs在成脂、成软骨和成骨分化能力方面的差异。来自年轻供体的细胞与组织培养聚苯乙烯的粘附性更好，并且比来自年老动物的细胞增殖更多。各组的成软骨和成骨潜能随着年龄的增长而下降，而成脂肪分化仅在年龄最大的供体细胞中下降。由于传代，还观察到来自最年轻供体的骨髓间充质干细胞成骨和来自最年长供体的细胞成软骨的分化潜力显著降低。年龄的增加和传代次数的增加对BMSC分化潜力都有谱系依赖的影响。此外，供体年龄和细胞传代之间存在明显的相互作用，未来在开发用于临床的细胞疗法时必须考虑到这一点。
Bone marrow-derived mesenchymal stem cells (BMSCs) are a widely researched adult stem cell population capable of differentiation into various lineages. Because many promising applications of tissue engineering require cell expansion following harvest and involve the treatment of diseases and conditions found in an aging population, the effect of donor age and ex vivo handling must be understood in order to develop clinical techniques and therapeutics based on these cells. Furthermore, there currently exists little understanding as to how these two factors may be influenced by one another. Differences in the adipogenic, chondrogenic, and osteogenic differentiation capacity of murine MSCs harvested from donor animals of different age and number of passages of these cells were observed. Cells from younger donors adhered to tissue culture polystyrene better and proliferated in greater number than those from older animals. Chondrogenic and osteogenic potential decreased with age for each group, and adipogenic differentiation decreased only in cells from the oldest donors. Significant decreases in differentiation potentials due to passage were observed as well for osteogenesis of BMSCs from the youngest donors and chondrogenesis of the cells from the oldest donors. Both increasing age and the number of passages have lineage dependent effects on BMSC differentiation potential. Furthermore, there is an obvious interplay between donor age and cell passage that in the future must be accounted for when developing cell-based therapies for clinical use.
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DOI：
10.1152/ajpregu.2001.280.6.r1772
发表时间：
2001-06-01
影响因子：
2.8
作者：
Karagiannides, I;Tchkonia, T;Kirkland, JL
通讯作者：
Kirkland, JL
影响因子：
4.8
作者：
Bishop-Bailey, D;Warner, TD
通讯作者：
Warner, TD
影响因子：
3
作者：
Iwata, Yoshio;Morihara, Toru;Kubo, Toshikazu
通讯作者：
Kubo, Toshikazu
影响因子：
--
作者：
Mendes, SC;Tibbe, JM;Van Blitterswijk, CA
通讯作者：
Van Blitterswijk, CA
影响因子：
3.1
作者：
Crisostomo, Paul R.;Wang, Meijing;Meldrum, Daniel R.
通讯作者：
Meldrum, Daniel R.