Human Brain Lectin: A Soluble Lectin That Binds Actin
Human Brain Lectin: A Soluble Lectin That Binds Actin
复制标题
人脑凝集素：结合肌动蛋白的可溶性凝集素
作者：
R. Joubert;M. Caron;V. Avellana‐Adalid;D. Mornet;D. Bladier
翻译后摘要：一种生物素化的探针用于检测内源性配体的人脑凝集素印迹人脑可溶性蛋白。在通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分离的脑提取物中的各种蛋白质中，有5种与生物素化探针反应。通过定期处理相同的提取物消除糖基化部分后，凝集素识别出Mr为43，000的单一条带。使用抗肌动蛋白抗体将该条带鉴定为肌动蛋白。这些结果通过生物素化的凝集素与纯化的肌动蛋白的结合得到证实。
Abstract: A biotinylated probe was used for detection of endogenous ligands of a human brain lectin on blotted human brain soluble proteins. Of the various proteins from brain extract resolved by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis, five reacted with the biotinylated probe. After elimination of saccharidic moieties by periodic treatment of the same extract, a single band with Mr∼ 43,000 was recognized by the lectin. This band was identified as actin using an anti‐actin antibody. These results were confirmed by binding of biotinylated lectin to purified actin.
MAP2 在树突棘中的分布及其与肌动蛋白的共定位。
影响因子：
3.6
作者：
Morales,M;Fifkova,E
通讯作者：
Fifkova,E