Hypoxia affects in vitro proliferation and differentiation of mouse corneal epithelial progenitor cell
Hypoxia affects in vitro proliferation and differentiation of mouse corneal epithelial progenitor cell
复制标题
缺氧影响小鼠角膜上皮祖细胞体外增殖和分化
DOI：
10.1007/s11626-013-9645-x
复制
发表时间：
2013-06
期刊：
影响因子：
--
通讯作者：
Liu, Zuguo
中科院分区：
文献类型：
--
作者：
Qin, Wenjuan;Xue, Yuhua;Li, Cheng;Liu, Zuguo
本研究旨在探讨低氧空运培养对小鼠角膜上皮祖细胞增殖和分化的影响。小鼠角膜上皮祖细胞系在常氧和低氧条件下空运培养，培养时间长达2wk。在常氧条件下，暴露于空气中可诱导小鼠角膜上皮细胞的过度增殖和异常表皮样分化，而在低氧条件下暴露于空气中，虽然我们观察到增殖增加，但异常分化被抑制。低氧阻止异常分化的机制可能涉及Wnt信号通路的下调，而Wnt信号通路在低氧气升技术培养的细胞中被抑制。结论：低氧通过阻断Wnt/β-Catenin信号通路，在促进角膜上皮细胞增殖的同时，防止分化异常。
This study was to investigate the proliferation and differentiation of mouse corneal epithelial progenitor cell in hypoxic airlift culture. Mouse corneal epithelial progenitor cell line progenitor cells were cultured under airlift with normoxic and hypoxic conditions for various durations up to 2 wk. Under normoxic conditions when exposed to air, the hyperproliferation and abnormal epidermal-like differentiation of mouse corneal epithelium was induced, whereas when exposed to air under hypoxic conditions, although we observed augmented proliferation, the abnormal differentiation was inhibited. The mechanism by which hypoxia prevents abnormal differentiation may involve downregulation of Wnt signaling pathways, which were inhibited in cells cultured with hypoxic airlift technique. In conclusion, hypoxia can prevent abnormal differentiation while enhancing the proliferation of corneal epithelial cells by blocking Wnt/β-catenin signaling pathway.
登录
查看更多内容
影响因子：
64.5
作者：
H. Clevers
通讯作者：
H. Clevers
影响因子：
158.5
作者：
Rama, Paolo;Matuska, Stanislav;Pellegrini, Graziella
通讯作者：
Pellegrini, Graziella
影响因子：
4.4
作者：
Whitlock, NA;Agarwal, N;Crosson, CE
通讯作者：
Crosson, CE
影响因子：
3.4
作者：
R. Yanai;Yang Liu;Ji-Ae Ko;T. Nishida
通讯作者：
R. Yanai;Yang Liu;Ji-Ae Ko;T. Nishida
影响因子：
6.2
作者：
Faleo, Gaetano;Neto, Joao Seda;Nakao, Atsunori
通讯作者：
Nakao, Atsunori