Presynaptic ultrastructural plasticity along CA3→CA1 axons during long-term potentiation in mature hippocampus.
Presynaptic ultrastructural plasticity along CA3→CA1 axons during long-term potentiation in mature hippocampus.
复制标题
DOI：
10.1002/cne.23384
复制
发表时间：
2013-12-01
影响因子：
2.5
通讯作者：
Harris, Kristen M.
中科院分区：
文献类型：
--
作者：
Bourne, Jennifer N.;Chirillo, Michael A.;Harris, Kristen M.
关键词：
在CA 1区的成熟海马，突触发生后30分钟内诱导的LTP，然而，2小时许多小树突棘丢失，和那些剩下的有较大的突触。然而，关于突触前囊泡和轴突终扣的相关变化知之甚少。在CA 1层辐射的轴突进行了评价与三维重建连续切片电子显微镜在30分钟和2小时后，诱导LTP的θ-突发刺激（TBS）。在2小时时，具有单个突触后伴侣的轴突终扣的频率减少了33%，完全对应于小树突棘（头部直径<0.45 μm）的33%损失。停靠的囊泡在30分钟时减少，然后在诱导LTP后2小时恢复到对照水平。到2小时，突触前囊泡池中的小突触囊泡总体较少。网格蛋白介导的内吞作用被用作标记的本地活动，和轴突终扣含有网格蛋白包被的坑表现出更明显的减少突触前囊泡在30分钟和2小时后诱导LTP相对于对照值。推定的运输包，确定为一个集群之间发生的突触终扣的轴突囊泡，稳定在30分钟，但显着减少2小时后，诱导LTP。APV阻断了这些作用，这表明轴突终扣和突触前囊泡的丢失依赖于NMDA受体的激活。这些结果表明，特定的突触前超微结构的变化补充突触后超微结构可塑性在LTP。
In area CA1 of the mature hippocampus, synaptogenesis occurs within 30 min after the induction of LTP; however, by 2 hr many small dendritic spines are lost, and those remaining have larger synapses. Little is known, however, about associated changes in presynaptic vesicles and axonal boutons. Axons in CA1 stratum radiatum were evaluated with three-dimensional reconstructions from serial section electron microscopy at 30 min and 2 hr after induction of LTP by theta-burst stimulation (TBS). The frequency of axonal boutons with a single postsynaptic partner was decreased by 33% at 2 hr, corresponding perfectly to the 33% loss specifically of small dendritic spines (head diameters <0.45 μm). Docked vesicles were reduced at 30 min and then returned to control levels by 2 hr following induction of LTP. By 2 hr there were fewer small synaptic vesicles overall in the presynaptic vesicle pool. Clathrin-mediated endocytosis was used as a marker of local activity, and axonal boutons containing clathrin-coated pits showed a more pronounced decrease in presynaptic vesicles at both 30 min and 2 hr after induction of LTP relative to control values. Putative transport packets, identified as a cluster of less than 10 axonal vesicles occurring between synaptic boutons, were stable at 30 min but markedly reduced by 2 hr after the induction of LTP. APV blocked these effects, suggesting that the loss of axonal boutons and presynaptic vesicles was dependent on NMDA receptor activation during LTP. These findings show that specific presynaptic ultrastructural changes complement postsynaptic ultrastructural plasticity during LTP.
登录
查看更多内容
影响因子：
5.3
作者：
Bayazitov, Ildar T.;Richardson, Robert J.;Zakharenko, Stanislav S.
通讯作者：
Zakharenko, Stanislav S.
DOI：
10.1016/s0736-5748(02)00015-1
发表时间：
2002-06-01
影响因子：
1.8
作者：
Dobrunz, LE
通讯作者：
Dobrunz, LE
影响因子：
16.2
作者：
Enoki, Ryosuke;Hu, Yi-ling;Fine, Alan
通讯作者：
Fine, Alan
DOI：
10.1007/bf01261506
发表时间：
1977-01-01
期刊：
JOURNAL OF NEUROCYTOLOGY
影响因子：
--
作者：
FIFKOVA, E;VANHARREVELD, A
通讯作者：
VANHARREVELD, A
影响因子：
64.8
作者：
BEKKERS, JM;STEVENS, CF
通讯作者：
STEVENS, CF