Contributions of O Island 48 to Adherence of Enterohemorrhagic Escherichia coli O157:H7 to Epithelial Cells In Vitro and in Ligated Pig Ileal Loops
Contributions of O Island 48 to Adherence of Enterohemorrhagic Escherichia coli O157:H7 to Epithelial Cells In Vitro and in Ligated Pig Ileal Loops
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O Island 48 在体外和结扎猪回肠环中对肠出血性大肠杆菌 O157:H7 粘附到上皮细胞的贡献
DOI：
10.1128/aem.00507-09
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发表时间：
2009-07
影响因子：
4.4
通讯作者：
Zhu, Jing
中科院分区：
文献类型：
--
作者：
Liu, Bianfang;Wheatcroft, Roger;Gyles, Carlton L.;Yin, Xianhua;Chambers, James R.;Zhu, Jing
大肠杆菌O岛48（OI-48）由编码尿素酶、亚碲酸盐抗性（Ter）和粘附素Iha和AIDA-1的三个功能基因簇组成。这些簇在肠出血性E.大肠杆菌（EHEC）O 157：H7感染情况不明。构建了这三个区域的缺失突变体，并评价了它们在体外和结扎的猪回肠环中粘附上皮细胞的能力。Ter基因簇的缺失降低了生物体粘附并在IPEC-J2和HEp-2细胞上形成大簇的能力。通过引入野生型ter基因来补充突变，恢复了粘附和大簇形成。在结扎的猪回肠袢中的测试显示Ter阴性突变体的定殖减少，但与野生型定殖相比差异不显著（26.4% ± 21.2%对40.1% ± 19.1%; P = 0.168）。OI-48 aidA基因缺失对体外或体内粘附均无影响。iha和ureC基因的缺失对体外粘附没有影响，但显着减少了肠出血性大肠杆菌O 157：H7在结扎的猪肠中的定植。这些数据表明，Ter，Iha和尿素酶可能有助于EHEC O 157：H7的致病性，通过促进病原体粘附到宿主肠上皮细胞。
ABSTRACT O island 48 (OI-48) of Escherichia coli consists of three functional gene clusters that encode urease, tellurite resistance (Ter), and putative adhesins Iha and AIDA-1. The functions of these clusters in enterohemorrhagic E. coli (EHEC) O157:H7 infection are unknown. Deletion mutants for these three regions were constructed and evaluated for their ability to adhere to epithelial cells in vitro and in ligated pig ileal loops. Deletion of the Ter gene cluster reduced the ability of the organism to adhere to and form large clusters on IPEC-J2 and HEp-2 cells. Complementation of the mutation by introducing the wild-type ter genes restored adherence and large-cluster formation. Tests in ligated pig ileal loops showed a decrease in colonization by the Ter-negative mutant, but the difference was not significant compared to colonization by the wild type (26.4% ± 21.2% versus 40.1% ± 19.1%; P = 0.168). The OI-48 aidA gene deletion had no effect on adherence in vitro or in vivo. Deletion of the iha and ureC genes had no effect on adherence in vitro but significantly reduced the colonization of EHEC O157:H7 in the ligated pig intestine. These data suggest that Ter, Iha, and urease may contribute to EHEC O157:H7 pathogenesis by promoting adherence of the pathogen to the host intestinal epithelium.
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影响因子：
--
作者：
Murphy KC;Campellone KG
通讯作者：
Campellone KG
影响因子：
3.2
作者：
R. Ménard;P. Sansonetti;C. Parsot
通讯作者：
R. Ménard;P. Sansonetti;C. Parsot
DOI：
10.1016/b978-0-12-384939-7.10005-5
发表时间：
2012-02-29
期刊：
Principles of Molecular Virology
影响因子：
--
作者：
Cann AJ
通讯作者：
Cann AJ
DOI：
10.1038/nbt1183-784
发表时间：
1983-01-01
期刊：
BIO-TECHNOLOGY
影响因子：
--
作者：
SIMON, R;PRIEFER, U;PUHLER, A
通讯作者：
PUHLER, A
影响因子：
3.1
作者：
Gunzer, F;Bohn, U;Donohue-Rolfe, A
通讯作者：
Donohue-Rolfe, A