Research on the Impact and Mechanism for the Inhibition of Micrococcus Catalase Activity by Typical Tetracyclines.
Research on the Impact and Mechanism for the Inhibition of Micrococcus Catalase Activity by Typical Tetracyclines.
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典型四环素类药物抑制微球菌过氧化氢酶活性的影响及机制研究
DOI：
10.1155/2020/5085369
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发表时间：
2020
影响因子：
--
通讯作者：
Zong W
中科院分区：
文献类型：
--
作者：
Ren L;Wang Q;Du Y;Xu P;Zong W
抗生素作为一种以生物酶为靶点的潜在抑制剂，可能对生化处理过程产生一定的影响。以微球菌过氧化氢酶（CAT）为靶分子，研究其对典型四环素类抗生素的影响及抑制机制。毒性实验表明，TC对CAT的抑制作用大小顺序为四环素>金霉素>土霉素>强力霉素。利用荧光光谱和莫伊分子模拟技术，探讨TC对CAT的抑制作用机制。荧光分析表明，TC以静态猝灭方式猝灭CAT的荧光信号。结合毒性数据，推测TC与催化活性中心结合，从而抑制CAT，分子模拟数据进一步验证了上述推测。与莫伊软件推荐的TC与非催化中心形成的化合物相比，TC与CAT催化中心结合时，化合物的结合面积增大，能量变化显著。IBM SPSS统计显示TC毒性与O 13 → Glu 252、O 1 ← Arg 195、O 6 → Asp 249等氢键正相关，与O 10 →Pro363、O 10 → Lys 455、O 12 → Asn 127等氢键负相关。TC毒性与N4-Glu 252离子键呈正相关，与N4-Asp 379离子键呈负相关。上述关键位点的氢键和离子键与TC对CAT的抑制作用密切相关。
As potential inhibitors target to biological enzymes, antibiotics may have certain impacts on the biochemical treatment process. With micrococcus catalase (CAT) served as the target molecule, the impact and inhibition mechanism for typical tetracyclines (TCs) were evaluated. Toxicity experiments showed that TCs had significant inhibition on CAT in the sequence of tetracycline>chlortetracycline>oxytetracycline>doxycycline. To clarify the inhibition mechanism between TCs and CAT which was explored with the assistance of fluorescence spectroscopy and MOE molecule simulation. According to fluorescence analysis, TCs quenched the fluorescence signal of CAT by the mode of static quenching. Combined with toxicity data, it could be presumed that TCs combined with the catalytic active center and thus inhibited CAT. Above presumption was further verified by the molecular simulation data. When TCs combined with the catalytic center of CAT, the compounds have increased combination areas and prominent energy change (compared with the compounds formed by TCs and noncatalytic center recommend by MOE software). IBM SPSS statistics showed that TC toxicity positively correlated with the hydrogen bonds such as O13→Glu252, O1←Arg195, and O6→Asp249, but negatively correlated with the hydrogen bonds such as O10→Pro363, O10→Lys455, and O12 → Asn127. TC toxicity also positively correlated with the ion bonds ofN4-Glu252, but negatively correlated with the ion bonds of N4-Asp379. Hydrogen bonds and ion bonds for above key sites were closely related to the inhibition effect of TCs on CAT.
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影响因子：
8.9
作者：
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通讯作者：
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影响因子：
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作者：
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作者：
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通讯作者：
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DOI：
10.1080/00103624.2011.528493
发表时间：
2011-01-01
影响因子：
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作者：
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通讯作者：
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