Stabilization and detection of natural left-handed Z-DNA.
Stabilization and detection of natural left-handed Z-DNA.
复制标题
天然左手 Z-DNA 的稳定和检测。
DOI：
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发表时间：
1983
影响因子：
4.4
通讯作者：
F. Azorín
中科院分区：
文献类型：
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作者：
A. Rich;A. Nordheim;F. Azorín
左旋Z-DNA更受嘌呤和嘧啶交替的核苷酸序列的青睐。描述了这种偏好的结构基础，以及稳定Z-DNA的因素。稳定Z-DNA最重要的生物因素是负超卷曲和Z-DNA结合蛋白。描述了通过使用特定抗体来确定形成Z-DNA的片段的核苷酸序列的实验。部分pBR322在负超卷曲时形成Z-DNA。类似地，SV40猴肿瘤病毒的三个片段形成Z-DNA。它们位于转录增强子区，可能与Z-DNA结合蛋白一起，促进RNA聚合酶与染色质中的DNA连接。这是第一个表明Z-DNA在转录调控中发挥作用的具体例子。
Left-handed Z-DNA is favored by nucleotide sequences with alternating purines and pyrimidines. The structural basis of this preference is described, as well as the factors which stabilize Z-DNA. The most important biological factors stabilizing Z-DNA are negative supercoiling and Z-DNA binding proteins. Experiments are described which make it possible to determine the nucleotide sequences of segments which form Z-DNA through the use of specific antibodies. Parts of the plasmid pBR322 form Z-DNA upon negative supercoiling. Similarly, three segments of the SV40 simian tumor virus form Z-DNA. They are located in the transcriptional enhancer region and may function, together with Z-DNA binding proteins, in facilitating the attachment of RNA polymerase to the DNA in chromatin. This is the first specific example suggesting a role of Z-DNA in regulating transcription.
DOI：
10.1073/pnas.78.6.3546
发表时间：
1981-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
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作者：
LAFER, EM;MOLLER, A;RICH, A
通讯作者：
RICH, A