A guaninine nucleotide exchange factor is a component of the meiotic spindle pole body in Schizosaccharomyces pombe.
A guaninine nucleotide exchange factor is a component of the meiotic spindle pole body in Schizosaccharomyces pombe.
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DOI：
10.1091/mbc.e09-10-0842
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发表时间：
2010-04-01
影响因子：
3.3
通讯作者：
Neiman AM
中科院分区：
文献类型：
--
作者：
Yang HJ;Neiman AM
Spo13 is a sporulation-specific subunit of the Schizosaccharomyces pombe spindle pole body. We show that Spo13 acts specifically as an exchange factor for the Rab GTPase Ypt2 and that this activity is essential for spore formation. The meiotic spindle pole body may promote membrane assembly by stimulating the activity of a Rab GTPase. Spore morphogenesis in yeast is driven by the formation of membrane compartments that initiate growth at the spindle poles during meiosis II and grow to encapsulate daughter nuclei. Vesicle docking complexes, called meiosis II outer plaques (MOPs), form on each meiosis II spindle pole body (SPB) and serve as sites of membrane nucleation. How the MOP stimulates membrane assembly is not known. Here, we report that SpSpo13, a component of the MOP in Schizosaccharomyces pombe, shares homology with the guanine nucleotide exchange factor (GEF) domain of the Saccharomyces cerevisiae Sec2 protein. ScSec2 acts as a GEF for the small Rab GTPase ScSec4, which regulates vesicle trafficking from the late-Golgi to the plasma membrane. A chimeric protein in which the ScSec2-GEF domain is replaced with SpSpo13 is capable of supporting the growth of a sec2Δ mutant. SpSpo13 binds preferentially to the nucleotide-free form of ScSec4 and facilitates nucleotide exchange in vitro. In vivo, a Spspo13 mutant defective in GEF activity fails to support membrane assembly. In vitro specificity experiments suggest that SpYpt2 is the physiological substrate of SpSpo13. These results demonstrate that stimulation of Rab-GTPase activity is a property of the S. pombe MOP essential for the initiation of membrane formation.
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影响因子：
7.8
作者：
Nair, J;Muller, H;Peterson, M;Novick, P
通讯作者：
Novick, P
DOI：
10.1083/jcb.149.1.95
发表时间：
2000-04-03
期刊：
The Journal of cell biology
影响因子：
--
作者：
Elkind NB;Walch-Solimena C;Novick PJ
通讯作者：
Novick PJ
影响因子：
2.6
作者：
HIRATA, A;SHIMODA, C
通讯作者：
SHIMODA, C
DOI：
10.1007/bf00433721
发表时间：
1968-01-01
期刊：
MOLECULAR AND GENERAL GENETICS
影响因子：
--
作者：
BRESCH, C;MULLER, G;EGEL, R
通讯作者：
EGEL, R
影响因子：
3.3
作者：
Nakanishi, H;de los Santos, P;Neiman, AM
通讯作者：
Neiman, AM