Common features and interesting differences in transcriptional responses to secretion stress in the fungi Trichoderma reesei and Saccharomyces cerevisiae.
Common features and interesting differences in transcriptional responses to secretion stress in the fungi Trichoderma reesei and Saccharomyces cerevisiae.
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在真菌trichoderma resei和酿酒酵母中对分泌应激的转录反应的共同特征和有趣的差异。
DOI：
10.1186/1471-2164-7-32
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发表时间：
2006-02-22
期刊：
影响因子：
4.4
通讯作者：
Penttilä M
中科院分区：
文献类型：
--
作者：
Arvas M;Pakula T;Lanthaler K;Saloheimo M;Valkonen M;Suortti T;Robson G;Penttilä M
分泌应激是由分泌途径中蛋白质的折叠、修饰或运输受损引起的。在真菌中，响应分泌应激的基因诱导主要由未折叠蛋白反应（UPR）途径介导。本研究旨在揭示暴露于分泌应激的丝状真菌里氏木霉中发生的转录反应，并将其与酿酒酵母中发现的转录反应进行比较。通过 cDNA 消减和 cDNA 扩增片段长度多态性 (AFLP) 实验，对表达人组织纤溶酶原激活剂 (tPA) 的里氏木霉恒化培养物和用二硫苏糖醇 (DTT) 处理以防止正确的蛋白质折叠的分批生物反应器培养物进行分析。分离了对应于分泌应激下推测诱导的 457 个独特基因的 EST，并通过 Northern 分析证实了 60 个基因的表达模式。还在过度表达肌醇需求酶 1 (IREI) 蛋白（UPR 途径的传感器）的菌株中研究了这些基因的表达。为了将这些数据与酿酒酵母的数据进行比较，重新分析了已发表的关于酿酒酵母各种应激反应的转录组分析数据。在两种生物体中，响应分泌应激而上调的基因包括大量与分泌相关的基因。此外，对里氏木霉的分析揭示了 cpc1 转录因子基因和核小体基因的上调。 cpcA 和组蛋白基因 H4 的诱导也在用 DTT 处理的构巢曲霉培养物中被诱导。对分泌应激下诱导的基因的分析揭示了里氏木霉和丝状真菌应激反应的新特征。我们已经证明，除了先前相当清楚地表征的许多 ER 蛋白或分泌相关蛋白的基因诱导之外，还存在其他类型的反应。
Secretion stress is caused by compromised folding, modification or transport of proteins in the secretory pathway. In fungi, induction of genes in response to secretion stress is mediated mainly by the unfolded protein response (UPR) pathway. This study aims at uncovering transcriptional responses occurring in the filamentous fungi Trichoderma reesei exposed to secretion stress and comparing these to those found in the yeast Saccharomyces cerevisiae. Chemostat cultures of T. reesei expressing human tissue plasminogen activator (tPA) and batch bioreactor cultures treated with dithiothreitol (DTT) to prevent correct protein folding were analysed with cDNA subtraction and cDNA-amplified fragment length polymorphism (AFLP) experiments. ESTs corresponding to 457 unique genes putatively induced under secretion stress were isolated and the expression pattern of 60 genes was confirmed by Northern analysis. Expression of these genes was also studied in a strain over-expressing inositol-requiring enzyme 1 (IREI) protein, a sensor for the UPR pathway. To compare the data with that of S. cerevisiae, published transcriptome profiling data on various stress responses in S. cerevisiae was reanalysed. The genes up-regulated in response to secretion stress included a large number of secretion related genes in both organisms. In addition, analysis of T. reesei revealed up regulation of the cpc1 transcription factor gene and nucleosomal genes. The induction of the cpcA and histone gene H4 were shown to be induced also in cultures of Aspergillus nidulans treated with DTT. Analysis of the genes induced under secretion stress has revealed novel features in the stress response in T. reesei and in filamentous fungi. We have demonstrated that in addition to the previously rather well characterised induction of genes for many ER proteins or secretion related proteins also other types of responses exist.
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影响因子：
16
作者：
Harding, HP;Zhang, YH;Ron, D
通讯作者：
Ron, D
影响因子：
3.6
作者：
Al-Sheikh, H;Watson, AJ;Archer, DB
通讯作者：
Archer, DB
在真菌trichoderma resei和酿酒酵母中对分泌应激的转录反应的共同特征和有趣的差异。
影响因子：
4.4
作者：
Arvas M;Pakula T;Lanthaler K;Saloheimo M;Valkonen M;Suortti T;Robson G;Penttilä M
通讯作者：
Penttilä M
影响因子：
16
作者：
Casagrande, R;Stern, P;Ploegh, H
通讯作者：
Ploegh, H
影响因子：
3.1
作者：
Breyne, P;Dreesen, R;Zabeau, M
通讯作者：
Zabeau, M