Post-transcriptional control of class I MHC mRNA expression in adenovirus 12-transformed cells.
Post-transcriptional control of class I MHC mRNA expression in adenovirus 12-transformed cells.
复制标题
腺病毒 12 转化细胞中 I 类 MHC mRNA 表达的转录后控制。
作者：
R.T.M.J. Vaessen;A. Houweling;A. J. V. D. Eb
主要组织相容性复合体（MHC）的I类移植抗原的表达在由致癌的人腺病毒12（Ad 12）转化的细胞中被抑制。在一些自然发生的癌症中也观察到I类抗原表达的这种抑制，其有助于转化细胞的致瘤表型。在本研究中，I类基因的转录起始速率通过在三种不同类型的Ad 5-和Ad 12-转化细胞中的核运行测定来测量。转录率是相同的，在所有三个。还检查了I类信使RNA的稳定性，发现在所有三种细胞类型中是相同的。结果表明，在Ad 12转化的细胞中，抑制是由抑制细胞核中I类MHC信使RNA的转录后加工引起的。
Expression of the class I transplantation antigens of the major histocompatibility complex (MHC) is suppressed in cells transformed by the oncogenic human adenovirus 12 (Ad12). This suppression of class I antigen expression, which contributes to the tumorigenic phenotype of the transformed cells, has also been observed in some naturally occurring cancers. In the present study, the rate of transcription initiation of class I genes was measured by a nuclear run-on assay in Ad5- and Ad12-transformed cells of three different types. The rate of transcription was the same in all three. The stability of the class I messenger RNA was also examined and found to be the same in all three cell types. The results indicate that in Ad12-transformed cells the suppression is caused by an inhibition of the post-transcriptional processing of class I MHC messenger RNA in the nucleus.
影响因子：
11.2
作者：
D. J. Ruiter;Wilma Bergman;Kees Welvaart;Erik Scheffer;W. A. Vloten;Carlo Russo;S. Ferrane
通讯作者：
D. J. Ruiter;Wilma Bergman;Kees Welvaart;Erik Scheffer;W. A. Vloten;Carlo Russo;S. Ferrane