Genome-wide analysis of small RNAs reveals eight fiber elongation-related and 257 novel microRNAs in elongating cotton fiber cells.
Genome-wide analysis of small RNAs reveals eight fiber elongation-related and 257 novel microRNAs in elongating cotton fiber cells.
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DOI：
10.1186/1471-2164-14-629
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发表时间：
2013-09-17
期刊：
影响因子：
4.4
通讯作者：
Liu JY
中科院分区：
文献类型：
--
作者：
Xue W;Wang Z;Du M;Liu Y;Liu JY
MicroRNAs （miRNAs）和其他类型的小调控rna在植物转录后水平的基因表达调控中起着至关重要的作用。棉花是最重要的经济作物之一，但对mirna在棉纤维伸长过程中的作用知之甚少。在此，我们将高通量测序与计算分析相结合，鉴定了棉纤维伸长相关的小rna （sRNAs）。序列分析证实了79个已知的miRNA家族在拉长纤维细胞中表达，并鉴定出257个新的miRNA家族，这些miRNA主要来源于棉籽中相应的特异性位点。（G. raimondii）基因组。此外，mirnaome的比较显示，46个miRNA家族在整个伸长期间表达差异。重要的是，预测和实验验证的8个mirna靶点与纤维伸长有关，与钙和生长素信号转导、脂肪酸代谢、花青素合成和木质部组织分化具有明显的功能关系。此外，还鉴定了一个tasiRNA，并在体内实验验证了其靶点ARF4。这项研究不仅在棉纤维伸长细胞中发现了257个新的低丰度mirna，而且揭示了9个对纤维伸长重要的srna的潜在调控网络。延长棉纤维细胞中miRNA的鉴定和表征可能促进纤维miRNA调控机制的进一步研究，并提供miRNA在棉花中的重要性。
MicroRNAs (miRNAs) and other types of small regulatory RNAs play critical roles in the regulation of gene expression at the post-transcriptional level in plants. Cotton is one of the most economically important crops, but little is known about the roles of miRNAs during cotton fiber elongation. Here, we combined high-throughput sequencing with computational analysis to identify small RNAs (sRNAs) related to cotton fiber elongation in Gossypium hirsutum L. (G. hirsutum). The sequence analysis confirmed the expression of 79 known miRNA families in elongating fiber cells and identified 257 novel miRNAs, primarily derived from corresponding specific loci in the Gossypium raimondii Ulbr. (G. raimondii) genome. Furthermore, a comparison of the miRNAomes revealed that 46 miRNA families were differentially expressed throughout the elongation period. Importantly, the predicted and experimentally validated targets of eight miRNAs were associated with fiber elongation, with obvious functional relationships with calcium and auxin signal transduction, fatty acid metabolism, anthocyanin synthesis and the xylem tissue differentiation. Moreover, one tasiRNA was also identified, and its target, ARF4, was experimentally validated in vivo. This study not only facilitated the discovery of 257 novel low-abundance miRNAs in elongating cotton fiber cells but also revealed a potential regulatory network of nine sRNAs important for fiber elongation. The identification and characterization of miRNAs in elongating cotton fiber cells might promote the further study of fiber miRNA regulation mechanisms and provide insight into the importance of miRNAs in cotton.
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影响因子：
5.8
作者：
Gokani, SJ;Thaker, VS
通讯作者：
Thaker, VS
DOI：
10.1073/pnas.93.23.12768
发表时间：
1996-11-12
影响因子：
11.1
作者：
John, ME;Keller, G
通讯作者：
Keller, G
影响因子：
4.4
作者：
Kwak PB;Wang QQ;Chen XS;Qiu CX;Yang ZM
通讯作者：
Yang ZM
影响因子：
7.4
作者：
Baima, S;Possenti, M;Morelli, G
通讯作者：
Morelli, G
影响因子：
6.9
作者：
Li XR;Wang L;Ruan YL
通讯作者：
Ruan YL