Foramen lacerum impingement of trigeminal nerve root as a rodent model for trigeminal neuralgia.
Foramen lacerum impingement of trigeminal nerve root as a rodent model for trigeminal neuralgia.
复制标题
DOI：
10.1172/jci.insight.168046
复制
发表时间：
2023-06-08
期刊：
影响因子：
8
通讯作者：
Shen, Shiqian
中科院分区：
文献类型：
--
作者：
Ding, Weihua;Yang, Liuyue;Chen, Qian;Hu, Kun;Liu, Yan;Bao, Eric;Wang, Changning;Mao, Jianren;Shen, Shiqian
三叉神经痛（TN）是一种典型的神经痛性疼痛，具有独特的临床特征。在啮齿动物中建模TN是具有挑战性的。最近，我们发现啮齿类动物颅底的一个孔，撕裂孔，提供了直接进入三叉神经根的通道。使用这种方法，我们建立了三叉神经根破裂孔撞击（FLIT）模型，并观察到啮齿动物明显的疼痛样行为，包括阵发性不对称面部鬼脸，进食时头部倾斜，避免固体食物，以及缺乏木材咀嚼。FLIT模型概括了TN的关键临床特征，包括刺痛样行为和牙痛样行为。重要的是，当与三叉神经性疼痛模型（眶下神经慢性压迫性损伤[IoN-CCI]）相比时，FLIT模型与初级躯体感觉皮层（S1）中显著更高数量的c-Fos阳性细胞相关，揭示了FLIT模型中强大的皮层激活。在活体2光子钙成像，同步S1神经动力学存在于FLIT，但不是IoN-CCI模型，揭示了不同的疼痛模型中的皮质激活的差异。总之，我们的研究结果表明，FLIT是一个临床相关的啮齿动物模型的TN，可以促进疼痛的研究和治疗的发展。
Trigeminal neuralgia (TN) is a classic neuralgic pain condition with distinct clinical characteristics. Modeling TN in rodents is challenging. Recently, we found that a foramen in the rodent skull base, the foramen lacerum, provides direct access to the trigeminal nerve root. Using this access, we developed a foramen lacerum impingement of trigeminal nerve root (FLIT) model and observed distinct pain-like behaviors in rodents, including paroxysmal asymmetric facial grimaces, head tilt when eating, avoidance of solid chow, and lack of wood chewing. The FLIT model recapitulated key clinical features of TN, including lancinating pain–like behavior and dental pain–like behavior. Importantly, when compared with a trigeminal neuropathic pain model (infraorbital nerve chronic constriction injury [IoN-CCI]), the FLIT model was associated with significantly higher numbers of c-Fos–positive cells in the primary somatosensory cortex (S1), unraveling robust cortical activation in the FLIT model. On intravital 2-photon calcium imaging, synchronized S1 neural dynamics were present in the FLIT but not the IoN-CCI model, revealing differential implication of cortical activation in different pain models. Taken together, our results indicate that FLIT is a clinically relevant rodent model of TN that could facilitate pain research and therapeutics development.
登录
查看更多内容
影响因子：
16.2
作者：
Fino E;Yuste R
通讯作者：
Yuste R
影响因子：
9.9
作者：
Cruccu G;Finnerup NB;Jensen TS;Scholz J;Sindou M;Svensson P;Treede RD;Zakrzewska JM;Nurmikko T
通讯作者：
Nurmikko T
影响因子：
1.6
作者：
Ding, Weihua;Chen, Shuping;Yu, Wenhua
通讯作者：
Yu, Wenhua
影响因子：
25
作者：
Chen, Qian;Deister, Christopher A.;Feng, Guoping
通讯作者：
Feng, Guoping
影响因子：
4.1
作者：
Gonzalez-Sepulveda, Marta;Pozo, Oscar J.;Valverde, Olga
通讯作者：
Valverde, Olga