A common genomic framework for a diverse assembly of plasmids in the symbiotic nitrogen fixing bacteria.
A common genomic framework for a diverse assembly of plasmids in the symbiotic nitrogen fixing bacteria.
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DOI：
10.1371/journal.pone.0002567
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发表时间：
2008-07-02
期刊：
影响因子：
3.7
通讯作者：
González V
中科院分区：
文献类型：
--
作者：
Crossman LC;Castillo-Ramírez S;McAnnula C;Lozano L;Vernikos GS;Acosta JL;Ghazoui ZF;Hernández-González I;Meakin G;Walker AW;Hynes MF;Young JP;Downie JA;Romero D;Johnston AW;Dávila G;Parkhill J;González V
本工作的中心是两个密切相关的固氮共生细菌，根瘤菌豆科生物变种蚕豆3841和根瘤菌etli CFN 42的基因组比较。这些菌株保持了一个稳定的基因组核心，这也是其他根瘤菌物种所共有的，加上一个非常可变和重要的辅助成分。染色体是高度同线的，而质粒是由较少的同线块相关，并有镶嵌结构。质粒p42 f-pRL 12、p42 e-pRL 11和p42 b-pRL 9以及p42 c的大部分与pRL 10显示出相似，而共生质粒（p42 d和pRL 10）在结构上不相关，并且似乎遵循不同的进化路径。尽管净化选择作用于整个基因组，但辅助成分的进化速度更快。该组分主要由蛋白质组成，用于运输各种代谢物和外部来源的元素。目前的分析使我们能够得出结论，一个异质性和迅速多样化的质粒组共存于一个共同的基因组框架。
This work centres on the genomic comparisons of two closely-related nitrogen-fixing symbiotic bacteria, Rhizobium leguminosarum biovar viciae 3841 and Rhizobium etli CFN42. These strains maintain a stable genomic core that is also common to other rhizobia species plus a very variable and significant accessory component. The chromosomes are highly syntenic, whereas plasmids are related by fewer syntenic blocks and have mosaic structures. The pairs of plasmids p42f-pRL12, p42e-pRL11 and p42b-pRL9 as well large parts of p42c with pRL10 are shown to be similar, whereas the symbiotic plasmids (p42d and pRL10) are structurally unrelated and seem to follow distinct evolutionary paths. Even though purifying selection is acting on the whole genome, the accessory component is evolving more rapidly. This component is constituted largely for proteins for transport of diverse metabolites and elements of external origin. The present analysis allows us to conclude that a heterogeneous and quickly diversifying group of plasmids co-exists in a common genomic framework.
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