Microbial Rhodopsins: Diversity, Mechanisms, and Optogenetic Applications.
Microbial Rhodopsins: Diversity, Mechanisms, and Optogenetic Applications.
复制标题
DOI：
10.1146/annurev-biochem-101910-144233
复制
发表时间：
2017-06-20
影响因子：
16.6
通讯作者：
Spudich JL
中科院分区：
文献类型：
--
作者：
Govorunova EG;Sineshchekov OA;Li H;Spudich JL
微生物视紫红质是一类广泛存在于微生物世界的光活性亚视黄基蛋白。它们以其功能的多样性而闻名，使用共享的七跨膜螺旋设计和类似的光化学反应的变化来进行明显不同的光驱动能量和感觉转导过程。他们的研究有助于我们理解进化如何改变蛋白质支架以创造新的蛋白质化学，而它们作为用光控制膜电位的工具是光遗传学变革技术的基础。我们回顾了目前已知的功能，并对过去两年中发现的三种功能和结构不同的类型进行了更深入的评估：（ii）隐藻阳离子传导通道视紫红质（CCR），其在结构上不同于广泛用于神经激活的绿色藻类CCR，和（iii）酶视紫红质，具有光门控鸟苷酸环化酶或激酶活性，有望用于信号转导的光遗传学控制。
Microbial rhodopsins are a family of photoactive retinylidene proteins widespread throughout the microbial world. They are notable for their diversity of function, using variations of a shared seven-transmembrane helix design and similar photochemical reactions to carry out distinctly different light-driven energy and sensory transduction processes. Their study has contributed to our understanding of how evolution modifies protein scaffolds to create new protein chemistry, and their use as tools to control membrane potential with light is fundamental to the transformative technology of optogenetics. We review the currently known functions, and present more in-depth assessment of three functionally and structurally distinct types discovered over the past two years: (i) anion-conducting channelrhodopsins (ACRs) from cryptophyte algae, enabling efficient optogenetic neural suppression, (ii) cryptophyte cation-conducting channelrhodopsins (CCRs), structurally distinct from the green algae CCRs used extensively for neural activation, and (iii) enzymerhodopsins, with light-gated guanylylcyclase or kinase activity promising for optogenetic control of signal transduction.
登录
查看更多内容
影响因子：
62.1
作者：
Ernst, Oliver P.;Lodowski, David T.;Elstner, Marcus;Hegemann, Peter;Brown, Leonid S.;Kandori, Hideki
通讯作者：
Kandori, Hideki
DOI：
10.1073/pnas.1306826110
发表时间：
2013-08-13
影响因子：
11.1
作者：
Devine, Erin L.;Oprian, Daniel D.;Theobald, Douglas L.
通讯作者：
Theobald, Douglas L.
影响因子：
25
作者：
Boyden, ES;Zhang, F;Deisseroth, K
通讯作者：
Deisseroth, K
影响因子：
2.9
作者：
da Silva GF;Goblirsch BR;Tsai AL;Spudich JL
通讯作者：
Spudich JL
DOI：
10.1126/science.1252367
发表时间：
2014-04-25
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Berndt A;Lee SY;Ramakrishnan C;Deisseroth K
通讯作者：
Deisseroth K