Blueberry anthocyanins extract inhibits acrylamide-induced diverse toxicity in mice by preventing oxidative stress and cytochrome P450 2E1 activation
Blueberry anthocyanins extract inhibits acrylamide-induced diverse toxicity in mice by preventing oxidative stress and cytochrome P450 2E1 activation
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蓝莓花青素提取物通过防止氧化应激和细胞色素 P450 2E1 激活来抑制丙烯酰胺诱导的小鼠多种毒性
DOI：
10.1016/j.jff.2015.01.035
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发表时间：
2015-04
影响因子：
5.6
通讯作者：
Fang Chen
中科院分区：
文献类型：
--
作者：
Mengyao Zhao;Pengpu Wang;Yuchen Zhu;Xin Liu;Xiaosong Hu;Fang Chen
采用小鼠模型研究了蓝莓花青素提取物（BAE）对丙烯酰胺（AA）诱导的全身毒性、遗传毒性和生殖毒性的干预作用。与AA组相比，BAE恢复了AA诱导的血液学和血清化学的改变，显著降低了淋巴细胞和肝细胞的DNA损伤以及骨髓细胞微核的形成。同时，BAE提高了精子活力水平，降低了异常精子率。此外，BAE抑制aa诱导的ROS积累和谷胱甘肽（GSH）消耗，改善谷胱甘肽过氧化物酶（GPx）和谷胱甘肽s转移酶（GST）活性，提高GPx、GST和γ-谷氨酰半胱氨酸合成酶（γ-GCS）蛋白表达，抑制细胞色素P4502E1 （CYP2E1）蛋白表达。因此，BAE对aa毒性的干预机制可能与阻止aa诱导的氧化应激和激活CYP2E1有关。BAE作为营养保健品或膳食补充剂在化学预防AA毒性方面具有潜在的应用前景。
The intervention effects of blueberry anthocyanins extract (BAE) on the acrylamide (AA)-induced general toxicity, genotoxicity and reproductive toxicity were investigated in a mice model. Compared to the AA group, BAE restored the AA-induced alteration in haematology and serum chemistry, markedly decreased the DNA damage in lymphocyte and liver cells, as well as micronucleus formation in bone marrow cell. Meanwhile, BAE improved sperm motility levels and decreased abnormal sperm rate. Furthermore, BAE inhibited the AA-induced ROS accumulation and glutathione (GSH) depletion, ameliorated the activities of glutathione peroxidase (GPx) and glutathione-S-transferase (GST), improved the protein expression of GPx, GST and gamma-glutamyl cysteine synthase (γ-GCS), and inhibited the cytochrome P4502E1 (CYP2E1) protein expression. Thus, the intervention mechanism of BAE on the AA-induced toxicity possibly attributed to the prevention of AA-induced oxidative stress and CYP2E1 activation. BAE would have potential application for the chemoprevention of the AA toxicity as a nutraceutical or a dietary supplement.
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DOI：
10.1007/bf01986538
发表时间：
1973-06
期刊：
Agents and Actions
影响因子：
--
作者：
W. Schmid
通讯作者：
W. Schmid
影响因子：
4.1
作者：
da Costa, GG;Churchwell, MI;Doerge, DR
通讯作者：
Doerge, DR
影响因子：
6.1
作者：
Tareke, E;Rydberg, P;Törnqvist, M
通讯作者：
Törnqvist, M
DOI：
10.1016/j.mrgentox.2009.04.009
发表时间：
2009-05
期刊：
Mutation research
影响因子：
--
作者：
N. Oliveira;M. Pingarilho;Célia Martins;A. Fernandes;S. Vaz;V. Martins;J. Rueff;J. Gaspar
通讯作者：
N. Oliveira;M. Pingarilho;Célia Martins;A. Fernandes;S. Vaz;V. Martins;J. Rueff;J. Gaspar
影响因子：
4.1
作者：
Li, Yuan;Ma, Ruijing;Wang, Zhengfu
通讯作者：
Wang, Zhengfu