Fluorescence lifetime imaging ophthalmoscopy in glaucoma
Fluorescence lifetime imaging ophthalmoscopy in glaucoma
复制标题
荧光寿命成像检眼镜检查青光眼
DOI：
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发表时间：
2014
期刊：
影响因子：
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通讯作者：
M. Hammer
中科院分区：
文献类型：
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作者：
L. Ramm;S. Jentsch;R. Augsten;M. Hammer
为了在细胞水平上检测视网膜的变化，我们对原发性开角型青光眼进行了荧光寿命成像眼科检查（FLIO）。除了视网膜神经节细胞的损失外，代谢改变和组织重塑也是可能的[1,2]。采用改进的激光扫描检眼镜对43例青光眼患者（64.9±11.4岁）和54例健康对照（65.3±11.8岁，p= 0.85）眼底不同区域的自身荧光进行了观察。25例患者和39例对照组为失禁，18例患者和15例对照组为假性失禁。受试者无严重全身性疾病、糖尿病或眼部病变（既往白内障手术除外）。接受荧光香豆素衍生物抗凝治疗的患者被排除在外。所有调查均由当地机构审查委员会批准，并获得书面知情同意。由于不中断抗青光眼治疗，所有病例的眼压均低于22 mmHg。FLIO方法由Schweitzer等人描述。用短波长的光刺激眼底，会产生不同物质的荧光。因此，被测信号是由几个荧光团的和组成的。研究荧光衰减时间至少可以部分地分离单个荧光团[3,4]。利用荧光寿命成像检眼镜，可以将荧光衰减分配到三个分量，从而通过寿命参数τ1-3和振幅α1-3来描述。对于荧光的全局表征，计算所有衰减时间的振幅加权平均值τm。荧光激发波长为448 nm。在两个光谱通道（Ch1: 490 - 560 nm, Ch2: 560 - 700 nm）中捕获了发射，并分析了不同眼底区域的自身荧光特性（图1）。皮毛,
Dear Editor: With the aim to detect retinal changes at cellular level, fluorescence lifetime imaging ophthalmoscopy (FLIO) was conducted in primary open-angle glaucoma. In addition to the loss of retinal ganglion cells, metabolic alterations and tissue remodeling are conceivable [1, 2]. Using a modified laserscanning ophthalmoscope, the autofluorescence of different fundus regions was investigated in 43 glaucoma patients (64.9 ±11.4 years) and 54 healthy controls (65.3±11.8 years, p= 0.85). Twenty-five patients and 39 controls were phacic, and 18 patients and 15 controls were pseudophacic. Subjects without serious systemic diseases, diabetes mellitus, or ocular pathologies (except previous cataract surgery) were included. Patients under anti-coagulant therapy with fluorescent coumarin-derivatives were excluded. All investigations were approved by a local institutional review board, and written informed consent was obtained. As the anti-glaucomatous treatment was not interrupted, the intraocular pressure was lower than 22 mmHg in all cases. The FLIO method was described by Schweitzer et al. [3]. Exciting the fundus with light of short wavelengths leads to fluorescence of different substances. Therefore, the measured signal is composed of the sum of several fluorophores. Investigating the fluorescence decay time allows, at least partly, a separation of single fluorophores [3, 4]. Using the fluorescence lifetime imaging ophthalmoscope, the fluorescence decay can be allocated to three components, and thereby described through the lifetime parameters τ1–3 and amplitudes α1–3. For a global characterization of the fluorescence, the amplitude-weightedmean of all decay times, τm, is calculated. Fluorescence was excited with a wavelength of 448 nm. The emission was captured in two spectral channels (Ch1: 490– 560 nm, Ch2: 560–700 nm) and the autofluorescence properties in different fundus regions were analyzed (Fig. 1). Fur-
影响因子：
13.7
作者：
Chang EE;Goldberg JL
通讯作者：
Goldberg JL