Functional genomics in the wild: a case study with paper wasps shows challenges and prospects for RNA interference in ecological systems.
Functional genomics in the wild: a case study with paper wasps shows challenges and prospects for RNA interference in ecological systems.
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野生功能基因组学：纸黄蜂的案例研究显示了生态系统中 RNA 干扰的挑战和前景。
DOI：
10.1139/gen-2017-0066
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发表时间：
2018
期刊：
影响因子：
3.1
通讯作者：
Amy L. Toth
中科院分区：
文献类型：
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作者：
Susan A. Weiner;Amy C. Geffre;Amy L. Toth
RNA 干扰 (RNAi) 是一种通过敲低靶基因表达来评估基因功能的有用工具，并已成功应用于家庭和实验室生物体中。然而，RNAi在功能基因组学中的应用尚未完全扩展到自然环境中的生态模型生物中。在野外评估基因功能很重要，因为基因功能可能依赖于环境和背景。在这里，我们提出了一个利用 RNAi 评估野生纸黄蜂 Polistes metricus 基因功能的案例研究，以测试两个候选基因（NADH 脱氢酶 (NADHdh) 和类视黄醇和脂肪酸结合蛋白 (RfaBp)）在生殖种姓发育中的作用。先前的研究表明，与工蜂相比，这些基因在成为蜂后的幼虫中表达上调，但这种模式在实验室中被逆转，因此有必要进行实地研究。我们给田间菌落的幼虫口服 dsRNA，发现了短期敲低后 RfaBp 表达补偿性反弹的证据。我们还观察到，在 NADHdh dsRNA 处理的黄蜂中，脂质储备会出现类似工蜂的减少，这表明 NADHdh 在种姓发展中可能发挥作用。我们在现场生态模型生物中使用 RNAi 进行功能基因组学的挑战的背景下讨论了我们的结果。
RNA interference (RNAi) is a useful tool to assess gene function by knocking down expression of a target gene and has been used successfully in domestic and laboratory organisms. However, the use of RNAi for functional genomics has not fully extended into ecological model organisms in natural environments. Assessment of gene function in the wild is important because gene function can be environmentally and context dependent. Here, we present a case study using RNAi to assess gene function in wild paper wasps Polistes metricus, to test roles for two candidate genes (NADH dehydrogenase (NADHdh) and retinoid and fatty acid binding protein (RfaBp)) in the development of reproductive castes. Previous studies have shown that these genes are upregulated in larvae that become queens compared to workers, but this pattern was reversed in the laboratory, making field-based studies necessary. We orally administered dsRNA to larvae in field colonies and found evidence of a short-term knockdown followed by a compensatory rebound in expression for RfaBp. We also observed the predicted worker-like decrease in lipid stores in NADHdh dsRNA treated wasps, suggesting a possible role for NADHdh in caste development. We discuss our results in the context of challenges for using RNAi for functional genomics in ecological model organisms in the field.
影响因子：
2.2
作者：
Scott, Jeffrey G.;Michel, Kristin;Bartholomay, Lyric C.;Siegfried, Blair D.;Hunter, Wayne B.;Smagghe, Guy;Zhu, Kun Yan;Douglas, Angela E.
通讯作者：
Douglas, Angela E.