Towards the elements of successful insect RNAi.
Towards the elements of successful insect RNAi.
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DOI：
10.1016/j.jinsphys.2013.08.014
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发表时间：
2013-12
影响因子：
2.2
通讯作者：
Douglas, Angela E.
中科院分区：
文献类型：
--
作者：
Scott, Jeffrey G.;Michel, Kristin;Bartholomay, Lyric C.;Siegfried, Blair D.;Hunter, Wayne B.;Smagghe, Guy;Zhu, Kun Yan;Douglas, Angela E.
RNA干扰（RNA interference，RNAi）是一种序列特异性抑制基因表达的技术，为昆虫科学研究提供了巨大的机遇，尤其是在分析基因功能、控制害虫种群和减少病原体方面。大量关于昆虫RNAi的文献表明，RNAi的效率在不同物种、RNAi递送模式和靶向基因之间存在差异。转录抑制的持续时间也有变化。目前，我们预测特定基因/昆虫RNAi的理想实验策略的能力有限，因为我们不完全了解RNAi信号是否以及如何在昆虫细胞中扩增和传播。因此，最佳RNAi方案的开发是一个高度经验性的过程。通过系统分析昆虫RNAi机制的分子生理学基础，可以缓解这一限制。对RNAi在昆虫中的功能的概念性理解将促进RNAi在基因功能解剖中的应用，并快速跟踪RNAi在控制害虫和开发有效方法以保护有益昆虫和非昆虫节肢动物，特别是蜜蜂（Apis mellifera）和养殖的太平洋白色虾（Litopenaeus vannamei）免受病毒和寄生虫疾病的影响的应用。
RNA interference (RNAi), the sequence-specific suppression of gene expression, offers great opportunities for insect science, especially to analyze gene function, manage pest populations, and reduce disease pathogens. The accumulating body of literature on insect RNAi has revealed that the efficiency of RNAi varies between different species, the mode of RNAi delivery, and the genes being targeted. There is also variation in the duration of transcript suppression. At present, we have a limited capacity to predict the ideal experimental strategy for RNAi of a particular gene/insect because of our incomplete understanding of whether and how the RNAi signal is amplified and spread among insect cells. Consequently, development of the optimal RNAi protocols is a highly empirical process. This limitation can be relieved by systematic analysis of the molecular physiological basis of RNAi mechanisms in insects. An enhanced conceptual understanding of RNAi function in insects will facilitate the application of RNAi for dissection of gene function, and to fast-track the application of RNAi to both control pests and develop effective methods to protect beneficial insects and non-insect arthropods, particularly the honey bee (Apis mellifera) and cultured Pacific white shrimp (Litopenaeus vannamei) from viral and parasitic diseases.
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通过双链RNA的腹腔内注射，成年蜜蜂中卵黄蛋白基因功能的破坏。
影响因子：
3.5
作者：
通讯作者：
--
影响因子：
4.2
作者：
Campbell CL;Keene KM;Brackney DE;Olson KE;Blair CD;Wilusz J;Foy BD
通讯作者：
Foy BD
影响因子：
3.8
作者：
Arimatsu, Yuji;Kotani, Eiji;Furusawa, Toshiharu
通讯作者：
Furusawa, Toshiharu
DOI：
10.1073/pnas.0504982102
发表时间：
2005-08-09
影响因子：
11.1
作者：
Arakane, Y;Muthukrishnan, S;Kramer, KJ
通讯作者：
Kramer, KJ
影响因子：
2.2
作者：
Allen, Margaret L.;Walker, William B., III
通讯作者：
Walker, William B., III