Quorum quenching enzymes and their effects on virulence, biofilm, and microbiomes: a review of recent advances.
Quorum quenching enzymes and their effects on virulence, biofilm, and microbiomes: a review of recent advances.
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DOI：
10.1080/14787210.2020.1794815
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发表时间：
2020-12
影响因子：
5.7
通讯作者：
Elias M
中科院分区：
文献类型：
--
作者：
Sikdar R;Elias M
许多细菌的行为是由一种依赖于细胞密度的机制调节的，这种机制被称为群体感应（QS）。QS依赖于细菌细胞之间的通信，使用被称为自诱导剂的扩散信号分子。QS调节代谢、毒力和生物膜形成等生理过程。群体猝灭（Quorum Quenching， QQ）是利用化学或酶的手段来抑制QS，从而抵消QS所调节的行为。我们研究了细菌物种中主要的、不同的QS机制，特别强调了ahl介导的QS。我们还讨论了研究QS干扰的关键体外和体内系统。此外，我们强调了有希望的发展，如所使用的酶猝灭剂的底物偏好，在干扰QS对抗细菌毒力的应用中。通过最近分离的高度稳定的群体猝灭酶和/或分子工程的努力，干扰QS的影响最近在传统的单物种培养模型之外进行了评估。复杂微生物群落的信号中断被证明会导致复杂微生物行为的破坏和种群结构的变化。这些新发现，以及未来的研究，可能会导致关于QS的传统观点发生重大变化。
Numerous bacterial behaviors are regulated by a cell-density dependent mechanism known as Quorum Sensing (QS). QS relies on communication between bacterial cells using diffusible signaling molecules known as autoinducers. QS regulates physiological processes such as metabolism, virulence and biofilm formation. Quorum Quenching (QQ) is the inhibition of QS using chemical or enzymatic means to counteract behaviors regulated by QS. We examine the main, diverse QS mechanisms present in bacterial species, with a special emphasis on AHL-mediated QS. We also discuss key in vitro and in vivo systems in which interference in QS was investigated. Additionally, we highlight promising developments, such as the substrate preference of the used enzymatic quencher, in the application of interference in QS to counter bacterial virulence. Enabled via the recent isolation of highly stable quorum quenching enzymes and / or molecular engineering efforts, the effects of the interference in QS were recently evaluated outside of the traditional model of single species culture. Signal disruption in complex microbial communities was shown to result in the disruption of complex microbial behaviors, and changes in population structures. These new findings, and future studies, may result in significant changes in the traditional views about QS.
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影响因子：
4.6
作者：
Bergonzi C;Schwab M;Naik T;Daudé D;Chabrière E;Elias M
通讯作者：
Elias M
影响因子：
4.9
作者：
Chow, Jeng Yeong;Yang, Yuanyong;Yew, Wen Shan
通讯作者：
Yew, Wen Shan
影响因子：
2.1
作者：
Biswa, Pramal;Doble, Mukesh
通讯作者：
Doble, Mukesh
影响因子：
4.8
作者：
Chow, Jeng Yeong;Xue, Bo;Yew, Wen Shan
通讯作者：
Yew, Wen Shan
影响因子：
2.9
作者：
Chowdhary, Puneet K.;Keshavan, Neela;Haines, Donovan C.
通讯作者：
Haines, Donovan C.