Cytokine mRNA expression in painful radiculopathy.
Cytokine mRNA expression in painful radiculopathy.
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DOI：
10.1016/j.jpain.2008.07.008
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发表时间：
2009-01
期刊：
影响因子：
--
通讯作者：
Winkelstein BA
中科院分区：
文献类型：
--
作者：
Rothman SM;Huang Z;Lee KE;Weisshaar CL;Winkelstein BA
炎性细胞因子促进腰椎神经根病。暂时性宫颈损伤的细胞因子的调节，有或没有持久的炎症，仍然有待确定。大鼠C7神经根受到压缩（10 gf）、铬肠线暴露（chr）或其组合（10 gf +chr）。伤后1h取同侧C7脊髓和背根神经节（DRG），用实时荧光定量PCR检测IL-1β、IL-6和TNFα的表达。细胞因子mRNA增加后，所有三个损伤。10 gf +chr后DRG中TNFα mRNA显著高于假手术组（p=0.026）。脊髓IL-1β在10 gf和10 gf +chr后显著高于假手术组（p<0.024）; IL-6在10 gf +chr后显著高于假手术组（p<0.024）。在单独的研究中，在所有损伤范例中，在损伤时和6小时时再次给予可溶性TNFα受体。评估异常性疼痛并收获组织样品用于细胞因子PCR。10 gf和10 gf +chr的受体给药显著降低了异常性疼痛（p<0.005）。在第1天，对于10 gf +chr，治疗还显著降低DRG中的IL-1β和TNFα mRNA（p<0.028）。结果表明，颈神经根损伤中存在急性、强烈的细胞因子反应，其模式取决于损伤类型，DRG中TNFα mRNA的早期增加可能驱动短暂性颈损伤的疼痛相关信号传导。疼痛性颈神经根损伤后DRG和脊髓中的炎性细胞因子mRNA被定义。研究描述了TNFα在短暂性疼痛性神经根病中介导行为敏感性和炎性细胞因子的作用。结果概述了颈部疼痛中炎性细胞因子上调的早期反应。
Inflammatory cytokines contribute to lumbar radiculopathy. Regulation of cytokines for transient cervical injuries, with or without longer-lasting inflammation, remains to be defined. The C7 root in the rat underwent compression (10gf), chromic gut suture exposure (chr), or their combination (10gf+chr). Ipsilateral C7 spinal cord and dorsal root ganglia (DRG) were harvested at 1 hour after injury for real-time PCR analysis of IL-1β, IL-6 and TNFα. Cytokine mRNA increased following all three injuries. TNFα mRNA in the DRG was significantly increased over sham following 10gf+chr (p=0.026). Spinal IL-1β was significantly increased over sham following 10gf and 10gf+chr (p<0.024); IL-6 was significantly increased after 10gf+chr (p<0.024). In separate studies, the soluble TNFα receptor was administered at injury and again at 6 hours in all injury paradigms. Allodynia was assessed and tissue samples were harvested for cytokine PCR. Allodynia significantly decreased with receptor administration for 10gf and 10gf+chr (p<0.005). Treatment also significantly decreased IL-1β and TNFα mRNA in the DRG for 10gf+chr (p<0.028) at day 1. Results indicate an acute, robust cytokine response in cervical nerve root injury with varying patterns dependent on injury type, and that early increases in TNFα mRNA in the DRG may drive pain-related signaling for transient cervical injuries. Inflammatory cytokine mRNA in the DRG and spinal cord are defined following painful cervical nerve root injury. Studies describe a role for TNFα in mediating behavioral sensitivity and inflammatory cytokines in transient painful radiculopathy. Results outline an early response of inflammatory cytokine upregulation in cervical pain.
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影响因子：
3
作者：
DeLeo, JA;Winkelstein, BA
通讯作者：
Winkelstein, BA
影响因子：
5.3
作者：
Colburn, RW;Rickman, AJ;DeLeo, JA
通讯作者：
DeLeo, JA
影响因子：
3
作者：
Hubbard, RD;Winkelstein, BA
通讯作者：
Winkelstein, BA
DOI：
10.1016/s0169-328x(98)00257-5
发表时间：
1998-11-20
期刊：
MOLECULAR BRAIN RESEARCH
影响因子：
--
作者：
Arruda, JL;Colburn, RW;DeLeo, JA
通讯作者：
DeLeo, JA
影响因子：
3.6
作者：
Clark, Anna K.;Gentry, Clive;Malcangio, Marzia
通讯作者：
Malcangio, Marzia