Developmental expression and localization of MHC class I molecules in the human central nervous system
Developmental expression and localization of MHC class I molecules in the human central nervous system
复制标题
人类中枢神经系统中 MHC I 类分子的发育表达和定位
DOI：
10.1007/s00221-015-4345-2
复制
发表时间：
2015
影响因子：
2
通讯作者：
Jianqiong Zhang
中科院分区：
文献类型：
--
作者：
Aifeng Zhang;Hong Yu;Youji He;Yuqing Shen;Ying Zhang;Jiane Liu;Bo Fu;D. Lv;F. Miao;Jianqiong Zhang
最近的动物研究发现，主要组织相容性复合体（MHC） I类在中枢神经系统（CNS）中有神经元表达。然而，MHC I类在人类中枢神经系统中的发育表达谱尚不清楚。本研究采用免疫组织化学和免疫荧光技术，系统评价了MHC I类分子在人中枢神经系统发育过程中的表达和亚细胞定位。在20-33孕周（GW）期间，除了少数神经元外，大脑皮层中MHC I类表达相对缺失；然而，在耳蜗核和小脑皮层浦肯野细胞中表达迅速增加，而在黑质中表达缓慢。早在20 GW，并持续到33 GW，在脑干的一些核和神经纤维中也检测到表达，包括模糊性核、蓝斑核和孤立束。这些具有MHC I类蛋白表达的早期神经细胞后来发育成神经元形态。30-33 GW是神经元中MHC I类表达的重要时期，在此期间，发现MHC I类分子不仅在神经元细胞体和神经突中富集，而且在神经纤维和周围基质中也富集。成人脑组织中除脑血管内皮外未见表达。MHC I类分子在小脑浦肯野细胞、外侧膝状核和耳蜗核中显示出更大的突触后共定位。这些结果表明MHC I类分子在产前人类中枢神经系统中具有不同的时空表达模式，并有力地支持MHC I类分子在中枢神经系统发育和可塑性中发挥重要作用的观点。
Recent animal studies have found neuronal expression of major histocompatibility complex (MHC) class I in the central nervous system (CNS). However, the developmental expression profiles of MHC class I in human CNS remain unclear. Here, we systemically evaluate the expression and subcellular localization of MHC class I molecules during human CNS development using immunohistochemistry and immunofluorescence. Between the age of 20–33 gestational weeks (GW), MHC class I expression was relatively absent in the cerebral cortex with the exception of a few neurons; however, expression increased rapidly in the cochlear nuclei and in the cerebellar cortical Purkinje cells while increasing slowly in the substantia nigra. Expression was also detected in some nuclei and nerve fibers of the brain stem including the ambiguus nucleus, the locus coeruleus and the solitary tract as early as 20 GW and persisted through 33 GW. These early-stage neural cells with MHC class I protein expression later developed neuronal morphology. 30–33 GW is an important period of MHC class I expression in neurons, and during this period, MHC class I molecules were found to be enriched not only in neuronal cell bodies and neurites but also in nerve fibers and in the surrounding stroma. No expression was detected in the adult brain with exception of the cerebrovascular endothelium. MHC class I molecules displayed greater postsynaptic colocalization in cerebellar Purkinje cells, in the lateral geniculate nucleus and in the cochlear nuclei. These results demonstrate diverse spatiotemporal expression patterns for MHC class I molecules in the prenatal human CNS and strongly support the notion that MHC class I molecules play important roles in both CNS development and plasticity.
影响因子：
56.9
作者：
Huh, GS;Boulanger, LM;Shatz, CJ
通讯作者：
Shatz, CJ
自闭症和共享父母 HLA 抗原。
DOI：
10.1016/s0002-7138(09)60445-3
发表时间：
1985
期刊：
Journal of the American Academy of Child Psychiatry
影响因子：
--
作者：
Stubbs,EG;Ritvo,ER;Mason-Brothers,A
通讯作者：
Mason-Brothers,A