Anti-cell antibodies in exposed seronegative individuals with HIV type 1-neutralizing activity.
Anti-cell antibodies in exposed seronegative individuals with HIV type 1-neutralizing activity.
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暴露于血清阴性个体中的抗细胞抗体具有 HIV 1 型中和活性。
DOI：
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发表时间：
2000
影响因子：
1.5
通讯作者：
A. Siccardi
中科院分区：
文献类型：
--
作者：
L. Lopalco;C. Pastori;Antonio Cosma;S. Burastero;B. Capiluppi;E. Boeri;A. Beretta;A. Lazzarin;A. Siccardi
尽管反复接触HIV-1，但有些人仍然是血清阴性。本研究报告称，一部分暴露血清阴性（ESN）受试者的血清显示出HIV中和活性; 17份ESN血清中有5份中和了两种不同的HIV-1原代分离株，17份对照血清中无一份中和了两种不同的HIV-1原代分离株（中和滴度范围：1/20至1/60）。中和活性与4/4中和ESN血清的IgG部分相关。此外，在11/17和9/17的ESN血清（但没有对照血清），我们发现抗体对HLA I类和CD 4，分别。ESN血清之一（EU 22）有效中和了来自血清阳性伴侣的主要病毒，并显示出良好的广泛交叉反应性中和。来自EU 19和EU 22的两种IgG组分在外周血单核细胞（PBMC）上的免疫吸附去除了病毒中和抗体。ESN状态与中和活性（p<0.05）、抗HLA抗体（p<0.0002）和抗CD 4抗体（p<0.001）之间的相关性具有统计学显著性。然而，中和活性与抗HLA或抗CD 4抗体之间无统计学显著相关性。因此，可以说，在某些个体中，暴露于HIV-1而没有血清转化与HIV中和抗体（不针对病毒抗原）和/或抗细胞自身抗体相关，这些抗体可能对感染/进入过程中涉及的细胞抗原具有特异性。
Despite repeated exposures to HIV-1, some individuals remain seronegative. This study reports that sera from a fraction of exposed seronegative (ESN) subjects showed HIV-neutralizing activity; 5 of 17 ESN sera and none of 17 controls neutralized two different HIV-1 primary isolates (range of neutralizing titers: 1/20 to 1/60). The neutralizing activity was associated with the IgG fraction of 4 of 4 neutralizing ESN sera. Moreover, in 11 of 17 and 9 of 17 ESN sera (but none of the control sera) we found antibodies against HLA class I and CD4, respectively. One of the ESN sera (EU22) neutralized efficiently the primary virus derived from the seropositive partner and showed a good broadly cross-reactive neutralization. Immunoadsorption of two IgG fractions from EU19 and EU22 on peripheral blood mononuclear cells (PBMC) removed virus-neutralizing antibodies. The correlations between the ESN status and neutralizing activity (p<0.05), anti-HLA antibodies (p<0.0002), and anti-CD4 antibodies (p<0.001) were statistically significant. However, there was no statistically significant correlation between neutralizing activity and either anti-HLA or anti-CD4 antibodies. It can therefore be said that exposure to HIV-1 without seroconversion is, in some individuals, associated with HIV-neutralizing antibodies (not directed against viral antigens) and/or with anti-cell autoantibodies, which are possibly specific for cellular antigens involved in the infection/entry process.
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一种静息细胞测定，用于改进抗体介导的 HIV 1 型初级分离株中和的检测。
DOI：
10.1089/aid.1995.11.1449
发表时间：
1995
期刊：
AIDS research and human retroviruses.
影响因子：
--
作者：
Zolla-Pazner,S;Sharpe,S
通讯作者：
Sharpe,S
影响因子：
158.5
作者：
PANTALEO, G;MENZO, S;FAUCI, AS
通讯作者：
FAUCI, AS
影响因子：
1.5
作者：
T. McInerney;N. Dimmock
通讯作者：
T. McInerney;N. Dimmock
影响因子：
6.4
作者：
CLERICI, M;GIORGI, JV;SHEARER, GM
通讯作者：
SHEARER, GM