Mutation of the Streptococcus gordonii Thiol-Disulfide Oxidoreductase SdbA Leads to Enhanced Biofilm Formation Mediated by the CiaRH Two-Component Signaling System.
Mutation of the Streptococcus gordonii Thiol-Disulfide Oxidoreductase SdbA Leads to Enhanced Biofilm Formation Mediated by the CiaRH Two-Component Signaling System.
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DOI：
10.1371/journal.pone.0166656
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Lee SF
中科院分区：
文献类型：
--
作者：
Davey L;Halperin SA;Lee SF
戈登链球菌是人类口腔生物膜的寄生菌。以前，我们发现了一种叫做SdbA的酶，它在S. gordonii。SdbA是硫醇-二硫键氧化还原酶，其催化分泌蛋白中的二硫键。令人惊讶的是，SdbA的失活导致增强的生物膜形成。在这项研究中，我们研究了ΔsdbA突变体形成生物膜的基础。结果表明，生物膜的形成是由CiaRH和ComDE双组分信号系统之间的相互作用介导的。虽然它不影响S.在gordonii亲本菌株中，CiaRH在ΔsdbA突变体中上调，并且其对于增强的生物膜表型是必需的。通过灭活CiaRH或通过加入感受态刺激肽（其产生被CiaRH活性阻断）来逆转生物膜表型。竞争试验表明，增强的生物膜表型也对应于小鼠口腔定植的增加。因此，SdbA、CiaRH和ComDE之间的相互作用影响体外和体内的生物膜形成。
Streptococcus gordonii is a commensal inhabitant of human oral biofilms. Previously, we identified an enzyme called SdbA that played an important role in biofilm formation by S. gordonii. SdbA is thiol-disulfide oxidoreductase that catalyzes disulfide bonds in secreted proteins. Surprisingly, inactivation of SdbA results in enhanced biofilm formation. In this study we investigated the basis for biofilm formation by the ΔsdbA mutant. The results revealed that biofilm formation was mediated by the interaction between the CiaRH and ComDE two-component signalling systems. Although it did not affect biofilm formation by the S. gordonii parent strain, CiaRH was upregulated in the ΔsdbA mutant and it was essential for the enhanced biofilm phenotype. The biofilm phenotype was reversed by inactivation of CiaRH or by the addition of competence stimulating peptide, the production of which is blocked by CiaRH activity. Competition assays showed that the enhanced biofilm phenotype also corresponded to increased oral colonization in mice. Thus, the interaction between SdbA, CiaRH and ComDE affects biofilm formation both in vitro and in vivo.
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