Lysophosphatidylcholine enhances superoxide anions production via endothelial NADH/NADPH oxidase.
Lysophosphatidylcholine enhances superoxide anions production via endothelial NADH/NADPH oxidase.
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溶血磷脂酰胆碱通过内皮 NADH/NADPH 氧化酶增强超氧阴离子的产生。
DOI：
10.5551/jat1994.7.238
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发表时间：
2000
影响因子：
4.4
通讯作者：
M. Yokoyama
中科院分区：
文献类型：
--
作者：
S. Takeshita;N. Inoue;D. Gao;Y. Rikitake;S. Kawashima;R. Tawa;H. Sakurai;M. Yokoyama
包括超氧阴离子（O2(-)）在内的活性氧（ROS）在动脉粥样硬化中起关键作用，内皮细胞具有产生ROS的能力。为了研究ROS的酶促来源和溶血磷脂酰胆碱（一种致动脉粥样硬化脂质）的作用，我们采用荧光素增强化学发光（CL）法和电子自旋共振（ESR）法测定了培养的牛主动脉内皮细胞（baec）的ROS生成。BAEC匀浆具有NADH/NADPH氧化酶活性。微载体珠上培养的baec在基础条件下产生O2（-）。二苯碘（DPI）对NADH/ NADPH氧化酶的抑制作用显著减弱了O2（-）的产生，而其他氧化酶的抑制剂则没有抑制作用。虽然LPC使O2（-）的生成增加了约3.1倍，但其作用被DPI抑制。酪氨酸激酶抑制剂显著减弱lpc诱导的O2（-）产生。与DMPO的ESR表明，LPC增加了DMPO-羟基加合物的形成，并呈剂量和时间依赖性。这些数据表明，内皮细胞中O2（-）的基础产生主要由NADH/NADPH氧化酶系统介导，LPC通过酪氨酸激酶依赖途径激活该氧化酶以增强O2（-）的产生。LPC对ROS生成的增强可能与其致动脉粥样硬化特性有关。
Reactive oxygen species (ROS) including superoxide anions (O2(-)) play a key role in atherogenesis, and endothelial cells have the ability to generate ROS. To investigate the enzymatic sources of ROS and the effects of lysophosphatidylcholine (LPC), an atherogenic lipid, we measured ROS production in cultured bovine aortic endothelial cells (BAECs) by the lucigenin-enhanced chemiluminescence (CL) method and electron spin resonance (ESR). BAEC homogenates had the enzymatic activity of NADH/NADPH oxidase. BAECs cultured on microcarrier beads generated O2(-) under basal conditions. The inhibition of NADH/ NADPH oxidase by diphenylene iodonium (DPI) significantly attenuated O2(-) production, whereas no inhibitors of other oxidases suppressed it. Although LPC enhanced O2(-) production approximately 3.1-fold, its action was suppressed by DPI. Tyrosine kinase inhibitors significantly attenuated LPC-induced O2(-) production. ESR with DMPO demonstrated that LPC increased the formation of the DMPO-hydroxyl adduct in dose- and time-dependent manners. These data suggest that the basal production of O2(-) in endothelial cells is mainly mediated by the NADH/NADPH oxidase system and that LPC activates this oxidase to enhance O2(-) production through a tyrosine kinase-dependent pathway. The enhancement of ROS production by LPC is probably involved in its atherogenic property.
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乳酸对小牛肺动脉张力的 O2 依赖性调节：H2O2 和 cGMP 的潜在作用。
DOI：
10.1152/ajplung.1993.264.2.l141
发表时间：
1993
期刊：
The American journal of physiology
影响因子：
--
作者：
Omar,HA;Mohazzab,KM;Mortelliti,MP;Wolin,MS
通讯作者：
Wolin,MS
影响因子：
15.9
作者：
MARUI, N;OFFERMANN, MK;MEDFORD, RM
通讯作者：
MEDFORD, RM
DOI：
10.1161/01.atv.14.6.1007
发表时间：
1994-06-01
期刊：
ARTERIOSCLEROSIS AND THROMBOSIS
影响因子：
--
作者：
OHARA, Y;PETERSON, TE;HARRISON, DG
通讯作者：
HARRISON, DG
影响因子：
3.9
作者：
Liochev, SI;Fridovich, I
通讯作者：
Fridovich, I
影响因子：
20.1
作者：
GRIENDLING, KK;MINIERI, CA;ALEXANDER, RW
通讯作者：
ALEXANDER, RW