SR-B1 and CD10 combined immunoprofile for differential diagnosis of metastatic clear cell renal cell carcinoma and clear cell carcinoma of the ovary.
SR-B1 and CD10 combined immunoprofile for differential diagnosis of metastatic clear cell renal cell carcinoma and clear cell carcinoma of the ovary.
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SR-B1和CD 10联合免疫检测鉴别诊断转移性肾透明细胞癌和卵巢透明细胞癌
DOI：
10.1007/s10735-021-09963-3
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发表时间：
2021-06
影响因子：
3.2
通讯作者：
Li F
中科院分区：
文献类型：
--
作者：
Jiang T;Diao X;Ding M;Niu X;Wang C;Qi Y;Jia W;Pang L;Hu W;Zou H;Li F
透明细胞肾癌(CcRCC)和卵巢透明细胞癌(CCOC)都具有明显的细胞质形态特征，仅凭形态很难区分转移性CcRCC和CCOC。目前，还没有有效的免疫组织化学标记物来区分这两种肿瘤。研究表明，ccRCC的胞浆清亮主要是由于胞浆中富含胆固醇的脂类引起的，而CCOC的胞浆清亮主要是由于胞浆糖原的积聚所致。目的：推测参与高密度脂蛋白摄取的清道夫受体B型1(SR-B1)蛋白在肾小管上皮细胞和肾小管上皮细胞中的表达可能存在差异，CD10在肾小管上皮细胞中的高表达可能有助于鉴别肾小管上皮细胞癌和慢性肾小管细胞癌。方法：应用有效的免疫组织化学标记物对90例肾透明细胞癌和31例卵巢癌进行鉴别诊断。结果：肾透明细胞癌中SR-B1和CD10的表达明显高于慢性肾癌。SR-B1和CD10在CCOC中均呈局灶性弱-中等强度染色，其染色范围和强度均明显低于CcRCC。SR-B1诊断肾细胞癌的敏感性为74.4%，特异性为83.9%。CD10诊断CCOC的敏感性和特异性分别为93.3%和80.6%。SR-B1( +)、CD10( +)联合免疫谱诊断肾细胞癌的特异度为93.5%。联合应用SR-B1(-)、CD10(-)免疫图谱诊断慢性萎缩性胃炎的特异度为93.3%。结论：SR-B1和CD10联合检测是鉴别诊断CcRCC和CCOC的有价值的工具。
Both clear cell renal carcinoma (ccRCC) and clear cell carcinoma of the ovary (CCOC) have a clear cytoplasmic morphological feature, hence it is difficult to identify metastatic ccRCC and CCOC by morphology alone. At present, there are no effective immunohistochemical markers to distinguish between these two tumors. Studies have shown that the clear cytoplasm of ccRCC is mainly caused by cholesterol-rich lipids in the cytoplasm, while that of CCOC is due to the accumulation of cytoplasmic glycogen. Objective: to hypothesize that the scavenger receptor class B-type 1 (SR-B1) protein responsible for HDL cholesterol uptake may be differentially expressed in ccRCC and CCOC, and high CD10 expression in the renal tubular epithelium may assist in distinguishing between ccRCC and CCOC. Methods: effective immunohistochemical markers were applied in 90 cases of renal clear cell carcinoma and 31 cases of ovarian cancer to distinguish between the two types of tumors.Result: SR-B1 and CD10 expression is significantly higher in ccRCC than CCOC. Both SR-B1 and CD10 exhibited focal weak-medium intensity staining in CCOC, and their staining extent and intensity were significantly lower than ccRCC. The sensitivity and specificity of SR-B1 for identifying ccRCC were 74.4% and 83.9%, respectively. The sensitivity and specificity of CD10 for identifying CCOC were 93.3% and 80.6%, respectively. The combined SR-B1( +) CD10( +) immunoprofile supports the diagnosis of ccRCC with a specificity of 93.5%. The combined SR-B1(-) CD10(-) immunoprofile supports the diagnosis of CCOC with a specificity of 93.3%. Conclusions: our findings demonstrate that the combination of SR-B1 and CD10 immunoprofiling is a valuable tool for differential diagnosis of ccRCC and CCOC.
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影响因子：
5.6
作者：
Carney, Erin M.;Banerjee, Priya;Netto, George J.
通讯作者：
Netto, George J.
影响因子：
2.6
作者：
Gulec, Umran Kucukgoz;Paydas, Semra;Gumurdulu, Derya
通讯作者：
Gumurdulu, Derya
DOI：
10.1097/pgp.0000000000000274
发表时间：
2016-09
期刊：
International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists
影响因子：
--
作者：
Köbel M;Rahimi K;Rambau PF;Naugler C;Le Page C;Meunier L;de Ladurantaye M;Lee S;Leung S;Goode EL;Ramus SJ;Carlson JW;Li X;Ewanowich CA;Kelemen LE;Vanderhyden B;Provencher D;Huntsman D;Lee CH;Gilks CB;Mes Masson AM
通讯作者：
Mes Masson AM
DOI：
10.3760/cma.j.issn.0529-5807.2016.09.006
发表时间：
2016-09-08
期刊：
Zhonghua bing li xue za zhi = Chinese journal of pathology
影响因子：
--
作者：
Huang, H J;Chen, X Y
通讯作者：
Chen, X Y
影响因子：
3.4
作者：
Kuhn, Elisabetta;Ayhan, Ayse
通讯作者：
Ayhan, Ayse