Population genetic variation in gene expression is associated with phenotypic variation in Saccharomyces cerevisiae.
Population genetic variation in gene expression is associated with phenotypic variation in Saccharomyces cerevisiae.
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DOI：
10.1186/gb-2004-5-4-r26
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发表时间：
2004
期刊：
影响因子：
12.3
通讯作者：
Eisen MB
中科院分区：
文献类型：
--
作者：
Fay JC;McCullough HL;Sniegowski PD;Eisen MB
这项研究比较了9个自然分离的酿酒酵母在有或没有硫酸铜的情况下的基因表达水平。他们对自然产生的特征的分子基础有了深入的了解。基因表达的遗传变异和自然界中可观察到的表型变异之间的关系尚不清楚。确定有多少表型与基因表达的差异有关，以及有多少基因表达差异与表型有关，这对于理解复杂性状的分子基础和进化非常重要。我们比较了9个自然分离的酿酒酵母在有或没有硫酸铜的情况下的基因表达水平。在这9个菌株中，有两个菌株的生长速度减慢，另外两个菌株在硫酸铜存在下呈铁锈色。我们确定了633个基因，这些基因在不同菌株之间的表达存在显著差异。在这些基因中，有20个与硫酸铜抗性相关，24个与锈色相关。这些基因的功能与它们的表达模式相结合，表明存在相关和致病的表达差异。但大多数差异表达基因与这两种表型都不相关，并且在有无硫酸铜的情况下表现出相同的表达模式。为了确定这些表达差异是否可能在其他环境条件下导致表型差异，我们研究了水通道蛋白基因AQY2的差异表达预测的一种表型--耐冻性。我们发现耐冻性与AQY2的表达有关。基因表达差异提供了对自然发生性状的分子基础的实质性洞察，并可用于预测与环境相关的表型变异。
This study compared levels of gene expression among nine natural isolates of Saccharomyces cerevisiae grown in the presence or absence of copper sulfate. Insights were gained into the molecular basis of naturally occurring traits. The relationship between genetic variation in gene expression and phenotypic variation observable in nature is not well understood. Identifying how many phenotypes are associated with differences in gene expression and how many gene-expression differences are associated with a phenotype is important to understanding the molecular basis and evolution of complex traits. We compared levels of gene expression among nine natural isolates of Saccharomyces cerevisiae grown either in the presence or absence of copper sulfate. Of the nine strains, two show a reduced growth rate and two others are rust colored in the presence of copper sulfate. We identified 633 genes that show significant differences in expression among strains. Of these genes, 20 were correlated with resistance to copper sulfate and 24 were correlated with rust coloration. The function of these genes in combination with their expression pattern suggests the presence of both correlative and causative expression differences. But the majority of differentially expressed genes were not correlated with either phenotype and showed the same expression pattern both in the presence and absence of copper sulfate. To determine whether these expression differences may contribute to phenotypic variation under other environmental conditions, we examined one phenotype, freeze tolerance, predicted by the differential expression of the aquaporin gene AQY2. We found freeze tolerance is associated with the expression of AQY2. Gene expression differences provide substantial insight into the molecular basis of naturally occurring traits and can be used to predict environment dependent phenotypic variation.
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影响因子：
5.3
作者：
Bray, NJ;Buckland, PR;O'Donovan, MC
通讯作者：
O'Donovan, MC
影响因子：
16
作者：
Frand, AR;Kaiser, CA
通讯作者：
Kaiser, CA
影响因子：
2.9
作者：
ASHIDA, J;HIGASHI, N;KIKUCHI, T
通讯作者：
KIKUCHI, T
影响因子：
4.8
作者：
Gross, C;Kelleher, M;Winge, DR
通讯作者：
Winge, DR
影响因子：
64.5
作者：
Huang, MX;Zhou, Z;Elledge, SJ
通讯作者：
Elledge, SJ