Body mass influences cortical bone mass independent of leptin signaling.
Body mass influences cortical bone mass independent of leptin signaling.
复制标题
DOI：
10.1016/j.bone.2008.10.058
复制
发表时间：
2009-03
期刊：
影响因子：
4.1
通讯作者：
Kalra, S. H.
中科院分区：
文献类型：
--
作者：
Iwaniec, U. T.;Dube, M. G.;Boghossian, S.;Song, H.;Helferich, W. G.;Turner, R. T.;Kalra, S. H.
人类的肥胖与骨量增加有关。瘦素是一种由脂肪细胞产生的激素，其功能是能量平衡的哨兵，并可能介导体重对骨骼的假定积极影响。我们在雄性C57 B1/6野生型（WT）和瘦素缺陷型ob/ob小鼠中进行了研究，以确定高脂肪摄入诱导的体重增加是否会增加骨量，如果是这样，这是否需要中枢瘦素信号传导。在喂食常规小鼠饮食的9周龄和24周龄WT小鼠中评价体重与骨量和结构之间的关系。通过微型计算机断层扫描分析股骨和腰椎。在随后的研究中，缓慢和快速生长的ob/ob小鼠下丘脑注射含有瘦素基因的重组腺相关病毒（rAAV-lep）或对照载体rAAV-GFP（绿色荧光蛋白）。小鼠维持常规对照饮食5或7周，然后再分成组，继续对照饮食或喂食高脂肪饮食（45%千卡来自脂肪）8周。在WT小鼠中，股骨和椎骨骨量与体重呈正相关（Pearson r = 0.65-0.88，取决于终点）。rAAV-lep治疗显著降低了体重（-61%），但增加了股骨长度。然而，在股骨远端和腰椎中，rAAV-lep治疗减少了松质骨体积/组织体积、小梁数量和小梁厚度，并增加了小梁间距。高脂肪饮食增加体重，与载体治疗无关。总股骨骨体积、长度、横截面体积和皮质体积和厚度在体重增加的小鼠中增加，与rAAV治疗无关。在股骨远端，体重增加对松质骨结构没有影响，也没有向量×体重相互作用。在WT小鼠中，体重增加导致椎体松质骨体积/组织体积增加（+33%）。体重增加对ob/ob小鼠松质椎骨质量的独立影响极小。总之，这些发现表明，增加体重对股骨皮质骨质量有积极的影响，这是独立的瘦素信号。
Obesity in humans is associated with increased bone mass. Leptin, a hormone produced by fat cells, functions as a sentinel of energy balance, and may mediate the putative positive effects of body mass on bone. We performed studies in male C57Bl/6 wild type (WT) and leptin-deficient ob/ob mice to determine whether body mass gain induced by high fat intake increases bone mass and, if so, whether this requires central leptin signaling. The relationship between body mass and bone mass and architecture was evaluated in 9-week-old and 24-week-old WT mice fed a regular mouse diet. Femora and lumbar vertebrae were analyzed by micro computed tomography. In subsequent studies, slowly and rapidly growing ob/ob mice were injected in the hypothalamus with a recombinant adeno-associated virus containing the leptin gene (rAAV-lep) or a control vector, rAAV-GFP (green fluorescent protein). The mice were maintained on a regular control diet for 5 or 7 weeks and then subdivided into groups and either continued on the control diet or fed a high fat diet (45% of kcal from fat) for 8 weeks. In the WT mice, femoral and vertebral bone mass was positively correlated with body mass (Pearson’s r = 0.65–0.88 depending on endpoint). rAAV-lep therapy dramatically decreased body mass (−61%) but increased femur length. However, in the distal femur and lumbar vertebra, rAAV-lep therapy reduced cancellous bone volume/tissue volume, trabecular number and trabecular thickness, and increased trabecular spacing. The high fat diet increased body mass, irrespective of vector treatment. Total femur bone volume, length, cross-sectional volume, and cortical volume and thickness were increased in mice with increased body mass, independent of rAAV treatment. In the distal femur, increased body mass had no effect on cancellous architecture and there were no vector × body mass interactions. In WT mice, increased body mass resulted in increased (+33%) vertebral cancellous bone volume/tissue volume. Increased body mass had minimal independent effect on cancellous vertebral bone mass in ob/ob mice. Taken together, these findings suggest that increased body mass has a positive effect on femur cortical bone mass that is independent of leptin signaling.
登录
查看更多内容
影响因子：
6.2
作者：
Baldock, Paul A.;Allison, Susan;Herzog, Herbert
通讯作者：
Herzog, Herbert
影响因子：
3
作者：
Boghossian, S;Lecklin, A;Kalra, SP
通讯作者：
Kalra, SP
影响因子：
6.2
作者：
Burge, Russel;Dawson-Hughes, Bess;Tosteson, Anna
通讯作者：
Tosteson, Anna
影响因子：
3.5
作者：
Asomaning, Kofi;Bertone-Johnson, Elizabeth R.;Pekow, Penelope S.
通讯作者：
Pekow, Penelope S.
影响因子：
4.8
作者：
Bagnasco, M;Dube, MG;Kalra, SP
通讯作者：
Kalra, SP