Expression of m1-type muscarinic acetylcholine receptors by parvalbumin-immunoreactive neurons in the primary visual cortex: a comparative study of rat, guinea pig, ferret, macaque, and human.
Expression of m1-type muscarinic acetylcholine receptors by parvalbumin-immunoreactive neurons in the primary visual cortex: a comparative study of rat, guinea pig, ferret, macaque, and human.
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单胞菌素 - 免疫反应性神经元在原发性视觉皮层中的M1型毒蕈碱乙酰胆碱受体的表达：大鼠，豚鼠，雪貂，猕猴和人类的比较研究。
DOI：
10.1002/cne.23456
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发表时间：
2014-04-01
影响因子：
2.5
通讯作者：
Reynolds, John H.
中科院分区：
文献类型：
--
作者：
Disney, Anita A.;Reynolds, John H.
关键词：
胆碱能神经调节是哺乳动物唤醒和注意方面的候选机制。我们之前已经报道过，猕猴初级视皮层（V1）中的胆碱能调节强烈靶向GABA能中间神经元，特别是猕猴V1中绝大多数小清蛋白免疫反应（PV）神经元表达m1型（哌仑西平敏感，Gq偶联）毒蕈碱ACh受体（m1AChR）。相反，先前的生理数据表明，大鼠PV神经元很少表达哌仑西平敏感的毒蕈碱AChR。为了进一步研究这一明显的物种差异的胆碱能效应的初级视觉皮层，我们进行了比较研究的m1AChRs的PV神经元在V1的大鼠，豚鼠，雪貂，猕猴和人类的表达。我们通过双重免疫荧光共聚焦显微镜观察PV和mAChR免疫反应性胞体，发现物种差异是深刻的;猕猴，人类和豚鼠中绝大多数（>75%）的PV免疫反应神经元表达m1AChR。相比之下，在大鼠中，只有约25%的PV人群对m1AChRs具有免疫反应性。我们的数据显示，虽然它们的频率比灵长类动物低得多，但大鼠PV神经元确实表达Gq偶联的毒蕈碱AChR，这似乎在以前的体外研究中未被检测到。诸如此类的数据是至关重要的，在确定的物种，代表足够的模型的能力，胆碱能系统调节抑制灵长类动物皮层。J. Comp.神经元522：986-1003，2014.© 2013威利期刊公司.
Cholinergic neuromodulation is a candidate mechanism for aspects of arousal and attention in mammals. We have reported previously that cholinergic modulation in the primary visual cortex (V1) of the macaque monkey is strongly targeted toward GABAergic interneurons, and in particular that the vast majority of parvalbumin‐immunoreactive (PV) neurons in macaque V1 express the m1‐type (pirenzepine‐sensitive, Gq‐coupled) muscarinic ACh receptor (m1AChR). In contrast, previous physiological data indicates that PV neurons in rats rarely express pirenzepine‐sensitive muscarinic AChRs. To examine further this apparent species difference in the cholinergic effectors for the primary visual cortex, we have conducted a comparative study of the expression of m1AChRs by PV neurons in V1 of rats, guinea pigs, ferrets, macaques, and humans. We visualize PV‐ and mAChR‐immunoreactive somata by dual‐immunofluorescence confocal microscopy and find that the species differences are profound; the vast majority (>75%) of PV‐ir neurons in macaques, humans, and guinea pigs express m1AChRs. In contrast, in rats only ∼25% of the PV population is immunoreactive for m1AChRs. Our data reveal that while they do so much less frequently than in primates, PV neurons in rats do express Gq‐coupled muscarinic AChRs, which appear to have gone undetected in the previousin vitrostudies. Data such as these are critical in determining the species that represent adequate models for the capacity of the cholinergic system to modulate inhibition in the primate cortex. J. Comp. Neurol. 522:986–1003, 2014. © 2013 Wiley Periodicals, Inc.
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影响因子：
64.8
作者：
Herrero, J. L.;Roberts, M. J.;Delicato, L. S.;Gieselmann, M. A.;Dayan, P.;Thiele, A.
通讯作者：
Thiele, A.
DOI：
10.1016/s0079-6123(03)45015-2
发表时间：
2004-01-01
期刊：
ACETYLCHOLINE IN THE CEREBRAL CORTEX
影响因子：
--
作者：
Hasselmo, ME;McGaughy, J
通讯作者：
McGaughy, J
影响因子：
3.3
作者：
Arnold, HM;Burk, JA;Bruno, JP
通讯作者：
Bruno, JP
影响因子：
2.5
作者：
Disney, Anita A.;Domakonda, Kunal V.;Aoki, Chiye
通讯作者：
Aoki, Chiye
DOI：
10.1073/pnas.1010356107
发表时间：
2010-09-07
影响因子：
11.1
作者：
Collins, Christine E.;Airey, David C.;Kaas, Jon H.
通讯作者：
Kaas, Jon H.