Epstein-Barr Virus Promotes Human Monocyte Survival and Maturation through a Paracrine Induction of IFN-α
Epstein-Barr Virus Promotes Human Monocyte Survival and Maturation through a Paracrine Induction of IFN-α
复制标题
Epstein-Barr 病毒通过旁分泌诱导 IFN-α 促进人类单核细胞存活和成熟
作者：
Shahram Salek;S. Lyons;J. Arrand
The role of monocytes and macrophages during EBV infection is not clear. The interaction of EBV with human monocytes was investigated in terms of cell survival and morphological and phenotypic changes to gain a better understanding of the role of these cells during EBV infection. We show that EBV infection of PBMCs rescues monocytes from undergoing spontaneous apoptosis and dramatically enhances their survival. Results obtained with heat-inactivated virus, neutralizing anti-EBV mAb 72A1 and recombinant gp350, suggest that enhancement of viability by EBV requires both infectious virus and interaction between gp350 and its receptor. IFN-α either secreted within 24 h from PBMCs upon infection with EBV or exogenously added to unstimulated monocytes inhibited spontaneous apoptosis, indicating that induction of IFN-α is an early important survival signal responsible for the delay in the apoptosis of monocytes. EBV infection also induced acute maturation of monocytes to macrophages with morphological and phenotypic characteristics of potent APCs. Monocytes exposed to EBV became larger in size with increased granularity and expressed considerably higher levels of membrane HLA classes I and II, ICAM-1, CD80, CD86, and CD40 compared with uninfected cultures. These observations provide the first immunoregulatory links among EBV, IFN-α, and monocyte survival and maturation and importantly raise the possibility that these cells may serve as a vehicle for the dissemination of the virus as well as being active participants in eliciting anti-EBV T cell responses during acute infection.
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影响因子：
11.2
作者：
Douglas D. Ross;Christopher C. Joneckis;José V. Ordóñez;Allison M. Sisk;Richard K. Wu;Anne W. Hamburger;Richard E. Nora
通讯作者：
Douglas D. Ross;Christopher C. Joneckis;José V. Ordóñez;Allison M. Sisk;Richard K. Wu;Anne W. Hamburger;Richard E. Nora
影响因子：
4.4
作者：
M. K. Balcewicz-Sablinska;Joseph Keane;H. Kornfeld;H. Remold
通讯作者：
M. K. Balcewicz-Sablinska;Joseph Keane;H. Kornfeld;H. Remold
影响因子：
4.4
作者：
Stella Redpath;Ana Angulo;N. R. Gascoigne;Peter Ghazal
通讯作者：
Stella Redpath;Ana Angulo;N. R. Gascoigne;Peter Ghazal
影响因子：
32.4
作者：
Randolph, GJ;Inaba, K;Muller, WA
通讯作者：
Muller, WA
影响因子：
4.4
作者：
D. Mangan;S. Wahl
通讯作者：
D. Mangan;S. Wahl