Three different human tumor cell lines contain different oncogenes
Three different human tumor cell lines contain different oncogenes
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三种不同的人类肿瘤细胞系含有不同的癌基因
DOI：
10.1016/0092-8674(81)90054-4
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发表时间：
1981
期刊：
影响因子：
64.5
通讯作者：
R. Weinberg
中科院分区：
文献类型：
--
作者：
M. Murray;B. Shilo;C. Shih;D. Cowing;Ho Wen Hsu;R. Weinberg
我们已经从结肠癌和膀胱癌细胞系以及早幼粒细胞白血病细胞系中获得了转染人DNA后的转化小鼠细胞灶。通过使用高度重复的人DNA序列探针，可以显示这些病灶含有大量的人DNA序列。来自原代病灶的细胞DNA可用于随后的转染循环，从而产生含有相对较少的人DNA的次级病灶。次级病灶似乎仅含有与转化表型相关的人类序列。一组特征性的DNA限制性片段被发现在来自每个肿瘤细胞系DNA的次级病灶中是共同的。比较来自三种不同人肿瘤细胞系的次级病灶中发现的共同DNA片段表明，这三种细胞系含有三种不同的转化基因。引言通过基因转移技术将可选择的表型转移到受体细胞的能力已经使得多种哺乳动物基因的传代成为可能（Wigler等，1978，1979 a，1980; Graf等人，1979年;刘易斯等人，1980年）。最近，这种方法已被应用于致癌转化的研究。我们实验室的研究人员（Shih等人，1979年，1981年）和其他人（库珀等人，一九八○年; Krontiris和库珀，1981）已经表明，来自非病毒转化细胞的DNA能够诱导正常小鼠成纤维细胞的转化。该领域的初步工作涉及通过DNA转染将3-甲基胆蒽转化的小鼠成纤维细胞的致癌表型转移至NIH/3 T3小鼠细胞（Shih等人，1979年）。通过基因转移程序成功传代这些性状表明，在这些细胞中存在DNA序列，其在转染时表现得像一些充分研究的病毒转化基因（Klein，1980）。此外，这项工作证明了转化DNA跨越组织和物种屏障的能力。例如，从人类癌细胞系中提取的DNA能够
We have obtained foci of transformed mouse cells after transfection of human DNA from colon and bladder carcinoma cell lines and a promyelocytic leukemia cell line. These foci can be shown to contain a large number of human DNA sequences by use of highly repetitive human DNA sequence probes. Cell DNA from primary foci can be used in a subsequent cycle of transfection resulting in secondary foci that contain relatively little human DNA. Secondary foci appear to contain only the human sequences proximal to those responsible for the transformed phenotype. A set of characteristic DNA restriction fragments is found in common among secondary foci derived from each tumor cell line DNA. Comparison of the common DNA fragments found in secondary foci derived from three different human tumor cell lines indicates that these three cell lines contain three different transforming genes. introductionThe ability to transfer a selectable phenotype to a recipient cell by gene-transfer techniques has made it possible to passage a variety of mammalian genes (Wigler et al., 1978, 1979a, 1980; Graf et al., 1979; Lewis et al., 1980). Recently, this approach has been applied to the study of oncogenic transformation. Investigators in our laboratory (Shih et al., 1979, 1981) and others (Cooper et al., 1980; Krontiris and Cooper, 1981) have shown that DNAs from nonvirally transformed cells are able to induce transformation of normal mouse fibroblasts. Initial work in this area involved the transfer of the oncogenic phenotype of 3-methylcholanthrene-transformed mouse fibroblasts to NIH/3T3 mouse cells by DNA transfection (Shih et al., 1979). The successful passaging of these traits via gene-transfer procedures suggests the presence, in these cells, of DNA sequences that behave upon transfection like some well studied viral transforming genes (Klein, 1980). Moreover, this work demonstrated the ability of the transforming DNA to act across tissue and species barriers. For example, DNA extracted from a human carcinoma cell line was able
人脱氧核糖核酸中单个散布重复序列家族的复性率研究。
影响因子：
2.9
作者：
Rinehart,FP;Ritch,TG;Deininger,PL;Schmid,CW
通讯作者：
Schmid,CW